Document Detail


Granulosa cells promote differentiation of cortical stromal cells into theca cells in the bovine ovary.
MedLine Citation:
PMID:  16914692     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Formation of a theca cell (TC) layer is an important physiologic event that occurs during early follicular development. Nevertheless, little is known concerning the nature and regulation of the formation of the TC layer during follicular growth. Using an established coculture system in this study, we examined the hypothesis that stromal cells differentiate into TCs during early follicular development and that this process involves interaction with granulosa cells (GCs). Ovarian stromal cells from the bovine ovarian cortex (S(C)) and medulla (S(M)) were cultured with or without GCs from small antral follicles. The presence of GCs increased the number of lipid droplets and mitochondria, and it stimulated androstenedione production in S(C) and S(M). However, luteinizing hormone/choriogonadotropin receptor (LHCGR) mRNA abundance and hCG-induced cAMP and androstenedione production were increased in S(C) but not in S(M) by the presence of GCs. The present results indicate that GCs are involved in the functional differentiation and the acquisition of LH responsiveness in stromal cells of the ovarian cortex. We suggest that GC-S(C) interaction is important in the formation of the TC layer during early follicular development, although the nature of this interaction remains to be determined.
Authors:
Makoto Orisaka; Kimihisa Tajima; Tetsuya Mizutani; Kaoru Miyamoto; Benjamin K Tsang; Shin Fukuda; Yoshio Yoshida; Fumikazu Kotsuji
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-08-16
Journal Detail:
Title:  Biology of reproduction     Volume:  75     ISSN:  0006-3363     ISO Abbreviation:  Biol. Reprod.     Publication Date:  2006 Nov 
Date Detail:
Created Date:  2006-10-23     Completed Date:  2007-02-26     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0207224     Medline TA:  Biol Reprod     Country:  United States    
Other Details:
Languages:  eng     Pagination:  734-40     Citation Subset:  IM    
Affiliation:
Department of Obstetrics and Gynecology, University of Fukui, Fukui 910-1193, Japan.
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MeSH Terms
Descriptor/Qualifier:
Androstenedione / secretion
Animals
Cattle
Cell Culture Techniques
Cell Differentiation
Cells, Cultured
Female
Granulosa Cells / physiology*
Luteinizing Hormone / physiology
Microscopy, Electron, Scanning
Microscopy, Electron, Transmission
Ovary / cytology*,  physiology,  ultrastructure
Stromal Cells / cytology,  physiology,  ultrastructure
Theca Cells / cytology*,  physiology
Chemical
Reg. No./Substance:
63-05-8/Androstenedione; 9002-67-9/Luteinizing Hormone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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