Document Detail


Granulosa cell ligand NPPC and its receptor NPR2 maintain meiotic arrest in mouse oocytes.
MedLine Citation:
PMID:  20947764     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Granulosa cells of mammalian Graafian follicles maintain oocytes in meiotic arrest, which prevents their precocious maturation. We show that mouse mural granulosa cells, which line the follicle wall, express natriuretic peptide precursor type C (Nppc) messenger RNA (mRNA), whereas cumulus cells surrounding oocytes express mRNA of the NPPC receptor NPR2, a guanylyl cyclase. NPPC increased cGMP levels in cumulus cells and oocytes and inhibited meiotic resumption in vitro. Meiotic arrest was not sustained in most Graafian follicles of Nppc or Npr2 mutant mice, and meiosis resumed precociously. Oocyte-derived paracrine factors promoted cumulus cell expression of Npr2 mRNA. Therefore, the granulosa cell ligand NPPC and its receptor NPR2 in cumulus cells prevent precocious meiotic maturation, which is critical for maturation and ovulation synchrony and for normal female fertility.
Authors:
Meijia Zhang; You-Qiang Su; Koji Sugiura; Guoliang Xia; John J Eppig
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Science (New York, N.Y.)     Volume:  330     ISSN:  1095-9203     ISO Abbreviation:  Science     Publication Date:  2010 Oct 
Date Detail:
Created Date:  2010-10-15     Completed Date:  2010-11-10     Revised Date:  2014-09-15    
Medline Journal Info:
Nlm Unique ID:  0404511     Medline TA:  Science     Country:  United States    
Other Details:
Languages:  eng     Pagination:  366-9     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Cumulus Cells / metabolism*
Cyclic AMP / metabolism
Cyclic GMP / metabolism
Female
Granulosa Cells / metabolism*
Intercellular Signaling Peptides and Proteins / metabolism
Ligands
Meiosis*
Mice
Models, Biological
Mutation
Natriuretic Peptide, C-Type / genetics,  metabolism*
Oocytes / physiology*
Ovarian Follicle / cytology
Protein Precursors / genetics,  metabolism*
RNA, Messenger / genetics,  metabolism
Receptors, Atrial Natriuretic Factor / genetics,  metabolism*
Signal Transduction
Grant Support
ID/Acronym/Agency:
HD21970/HD/NICHD NIH HHS; HD23839/HD/NICHD NIH HHS; R01 HD023839/HD/NICHD NIH HHS; R01 HD023839-22/HD/NICHD NIH HHS; R37 HD021970/HD/NICHD NIH HHS; R37 HD021970-25/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
0/Intercellular Signaling Peptides and Proteins; 0/Ligands; 0/Nppa protein, mouse; 0/Protein Precursors; 0/RNA, Messenger; 0/natriuretic peptide precursor type C, mouse; 127869-51-6/Natriuretic Peptide, C-Type; E0399OZS9N/Cyclic AMP; EC 4.6.1.2/Receptors, Atrial Natriuretic Factor; EC 4.6.1.2/atrial natriuretic factor receptor B; H2D2X058MU/Cyclic GMP
Comments/Corrections

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