Document Detail

Granulosa cell kinetics examined by bromodeoxyuridine in combination with colchicine in the mouse ovarian follicles.
MedLine Citation:
PMID:  9239519     Owner:  NLM     Status:  MEDLINE    
To elucidate the relationship between follicular growth and granulosa cell kinetics, ovarian histology was examined in mice treated with 5-bromo-2'-deoxyuridine (BrdU) or in combination with colchicine. In normal-cycling mice, active proliferation of the granulosa cells started in some follicles with diameters of more than 30 microns and all follicles more than 70 microns in diameter had already grown. Thus follicles between 30 and 70 microns were selected to start growing from those remaining in the resting phase. Although the granulosa cells synthesize DNA and mitosis took place throughout the epithelia in the follicles < 300 microns, such proliferating cells were distributed centripetally in follicles > 300 microns. In follicles measuring more than 400 microns in diameter DNA synthesis and cell division occurred in the inner epithelial layers after which, some of the divided cells moved to the outermost mural layer as fully differentiated cells to prepare for ovulation.
Y Shinohara; H Matsumoto; T Mori
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  In vivo (Athens, Greece)     Volume:  11     ISSN:  0258-851X     ISO Abbreviation:  In Vivo     Publication Date:    1997 May-Jun
Date Detail:
Created Date:  1997-09-09     Completed Date:  1997-09-09     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8806809     Medline TA:  In Vivo     Country:  GREECE    
Other Details:
Languages:  eng     Pagination:  249-52     Citation Subset:  IM    
Department of Biological Sciences, Graduate School of Science, University of Tokyo, Japan.
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MeSH Terms
Cell Division / drug effects,  physiology
DNA / biosynthesis
Gout Suppressants
Granulosa Cells / cytology*,  drug effects,  physiology*
Mice, Inbred ICR
Mitosis / physiology
Staining and Labeling
Reg. No./Substance:
0/Antimetabolites; 0/Gout Suppressants; 59-14-3/Bromodeoxyuridine; 64-86-8/Colchicine; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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