| Granulosa cell subtypes vary in response to oxidized low-density lipoprotein as regards specific lipoprotein receptors and antioxidant enzyme activity. | |
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MedLine Citation:
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PMID: 20444928 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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CONTEXT: The oxidized low-density lipoprotein (oxLDL) and its lectin-like oxLDL receptor-1 (LOX-1) are found in the follicular fluid and in granulosa cells. Lipoprotein receptors and antioxidant enzymes could differ in granulosa cell subtypes. OBJECTIVE: Our aim was to reveal cell-specific responses under oxLDL treatment. DESIGN AND SETTING: We conducted basic research at the Institute of Anatomy and the Clinic of Reproductive Medicine. PATIENTS: Women undergoing in vitro fertilization therapy participated in the study. MAIN OUTCOME MEASURES: Cultures of cytokeratin-positive/negative (CK(+)/CK(-)) granulosa cells and of cumulus cells were treated with 150 microg/ml oxLDL or native LDL under serum-free conditions for up to 36 h. Dead cells were determined by uptake of propidium iodide. LOX-1, toll-like receptor 4, and cluster of differentiation 36 (CD36) were examined in lysates by Western blots. The enzyme activities were determined in lysates and in supernatants. RESULTS: Under oxLDL treatment, predominantly CK(+) cells underwent nonapoptotic cell death. Receptors showed a cell-specific pattern of up-regulation: toll-like receptor 4 in CK(+) cells, LOX-1 in CK(-) cells, and CD36 in cumulus cells. An antioxidant ranking occurred: superoxide dismutase activity in CK(+) cells, total glutathione in CK(-) cells, and catalase activity in cumulus cells. The supernatants of oxLDL-treated CK(+) cell cultures contained more catalase activity than in controls, whereas a moderate increase was noted for glutathione peroxidase (GPx) in supernatants of CK(-) and cumulus cells. CONCLUSIONS: Catalase/GPx activity in the supernatants may be due to cell death or to secretion. Oxidative stress could be sensed by CK(+) cells and indicated by changes in catalase/GPx activity in the follicular fluid during ovarian disorders. |
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Authors:
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H Serke; J Bausenwein; J Hirrlinger; M Nowicki; C Vilser; P Jogschies; F A Hmeidan; V Blumenauer; K Spanel-Borowski |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-05-05 |
Journal Detail:
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Title: The Journal of clinical endocrinology and metabolism Volume: 95 ISSN: 1945-7197 ISO Abbreviation: J. Clin. Endocrinol. Metab. Publication Date: 2010 Jul |
Date Detail:
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Created Date: 2010-07-08 Completed Date: 2010-07-26 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0375362 Medline TA: J Clin Endocrinol Metab Country: United States |
Other Details:
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Languages: eng Pagination: 3480-90 Citation Subset: AIM; IM |
Affiliation:
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Institute of Anatomy, University of Leipzig, Liebigstrasse 13, D-04103 Leipzig, Germany. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Analysis of Variance Antigens, CD36 / metabolism Antioxidants / metabolism* Blotting, Western Cell Count Cells, Cultured Female Fluorescent Antibody Technique Glutathione / metabolism Granulosa Cells / metabolism* Humans Lipoproteins, LDL / metabolism* Receptors, LDL / metabolism* Scavenger Receptors, Class E / metabolism* Superoxide Dismutase / metabolism Toll-Like Receptor 4 / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Antigens, CD36; 0/Antioxidants; 0/Lipoproteins, LDL; 0/OLR1 protein, human; 0/Receptors, LDL; 0/Scavenger Receptors, Class E; 0/TLR4 protein, human; 0/Toll-Like Receptor 4; 70-18-8/Glutathione; EC 1.15.1.1/Superoxide Dismutase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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