Document Detail

Granulocyte-macrophage colony stimulating factor modulates the production of TNF alpha by differentiated U937 cells infected with Leishmania major.
MedLine Citation:
PMID:  10190115     Owner:  NLM     Status:  MEDLINE    
In this work, the production of tumor necrosis factor alpha (TNF alpha) during interaction of human phagocytes with the intracellular parasite Leishmania major was further investigated. The human monocytic cell line U937, differentiated with a combination of 1 alpha, 25 dihydroxyvitamin D3 (VD) and retinoic acid (RA), or with granulocyte macrophage colony stimulating factor (GM-CSF) was used. Differentiated U937 cells were infected with Leishmania major promastigotes, and TNF alpha was assayed in cell culture supernatants. It was found that the cytokine was produced only by U937 cells differentiated with VD/RA and further incubated with GM-CSF and LPS or interferon gamma (IFN gamma). L. major induced TNF alpha production only in the presence of GM-CSF. No direct relationship was found, however, between production of TNF alpha and resistance of differentiated U937 cells to infection with L. major.
A Arena; A B Capozza; F Marino; S Zummo; D Iannello; P Mastroeni
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The new microbiologica     Volume:  22     ISSN:  1121-7138     ISO Abbreviation:  New Microbiol.     Publication Date:  1999 Jan 
Date Detail:
Created Date:  1999-06-02     Completed Date:  1999-06-02     Revised Date:  2011-02-25    
Medline Journal Info:
Nlm Unique ID:  9516291     Medline TA:  New Microbiol     Country:  ITALY    
Other Details:
Languages:  eng     Pagination:  31-9     Citation Subset:  IM    
Istituto di Microbiologia, Facoltà di Medicina e Chirurgia Torre Biologica, Policlinico Universitario, Messina, Italy.
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MeSH Terms
Adjuvants, Immunologic / pharmacology*
Cell Differentiation / drug effects
Granulocyte-Macrophage Colony-Stimulating Factor / pharmacology*
Interferon-gamma / pharmacology
Leishmania major*
Lipopolysaccharides / pharmacology
Time Factors
Tretinoin / pharmacology
Tumor Necrosis Factor-alpha / metabolism*
U937 Cells / metabolism,  parasitology
Vitamin D / pharmacology
Reg. No./Substance:
0/Adjuvants, Immunologic; 0/Lipopolysaccharides; 0/Tumor Necrosis Factor-alpha; 1406-16-2/Vitamin D; 302-79-4/Tretinoin; 82115-62-6/Interferon-gamma; 83869-56-1/Granulocyte-Macrophage Colony-Stimulating Factor

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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