| Granulocyte colony-stimulating factor and stem cell factor improve contractile reserve of the infarcted left ventricle independent of restoring muscle mass. | |
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MedLine Citation:
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PMID: 16256866 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVES: We investigated whether granulocyte colony-stimulating factor (G-CSF) and stem cell factor (SCF) could promote myocardial regeneration after coronary artery occlusion and improve left ventricular (LV) function. BACKGROUND: Cytokine-induced mobilization of bone marrow stem cells in the heart may represent a promising strategy for replacing infarcted myocardium. METHODS: Sprague-Dawley rats were subjected to permanent coronary occlusion. A treated group (n = 19) received G-CSF (100 microg/kg) and SCF (25 microg/kg) subcutaneously, starting 2 h after surgery and continuing daily for an additional 4 days. Control rats (n = 21) received sterile water. The peripheral blood content in hematopoietic progenitor cells was analyzed. RESULTS: At eight weeks, LV angiograms (rest and dobutamine stress) and histologic analysis were performed. At rest, LV ejection fraction (LVEF) was 0.45 in controls and 0.52 in treated hearts (p = 0.16). For any infarct size, LVEF was greater in the treated group (p = 0.045). Under dobutamine stress, treated animals had smaller LV end-diastolic and -systolic volumes (0.37 +/- 0.04 ml and 0.16 +/- 0.03 ml) versus control animals (0.51 +/- 0.05 ml and 0.26 +/- 0.04 ml; p = 0.026 and 0.048) with a 7% improvement in ejection fraction. Scar thickness was 1.1 +/- 0.1 mm in treated hearts and 1.0 +/- 0.1 mm in controls (p = 0.36). Scar morphology was similar in both groups without obvious new muscle in the scar. CONCLUSIONS: Because we did not find evidence of new muscle cells in the infarct area, our conclusion is that G-CSF and SCF enhanced the LV functional reserve of the heart without replacing scar tissue. |
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Authors:
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Casilde Sesti; Sharon L Hale; Carolyn Lutzko; Robert A Kloner |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Journal of the American College of Cardiology Volume: 46 ISSN: 1558-3597 ISO Abbreviation: J. Am. Coll. Cardiol. Publication Date: 2005 Nov |
Date Detail:
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Created Date: 2005-10-31 Completed Date: 2005-12-29 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 8301365 Medline TA: J Am Coll Cardiol Country: United States |
Other Details:
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Languages: eng Pagination: 1662-9 Citation Subset: AIM; IM |
Affiliation:
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Heart Institute, Good Samaritan Hospital, Cardiovascular Division, Keck School of Medicine at the University of Southern California, Los Angeles, California 90017-2395, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Female Granulocyte Colony-Stimulating Factor / therapeutic use* Myocardial Contraction Myocardial Infarction / drug therapy*, pathology, physiopathology* Rats Rats, Sprague-Dawley Stem Cell Factor / therapeutic use* Ventricular Function, Left* |
| Grant Support | |
ID/Acronym/Agency:
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1R01-HLO73709/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Stem Cell Factor; 143011-72-7/Granulocyte Colony-Stimulating Factor |
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