| Granulocyte colony-stimulating factor receptor mediated cell growth and differentiation. | |
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MedLine Citation:
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PMID: 8704276 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Granulocyte colony-stimulating factor (G-CSF) can elicit different biological responses through interaction with its receptor (G-CSFR). A series of G-CSFR expressing clones were established from WEHI-3B D+ myelomonocytic leukemia cells and interleukin-3 (IL-3) dependent Ba/F3 preB cells by transduction of a G-CSFR cDNA. The overexpression of the G-CSFR from the expression vector promoted differentiation in WEHI-3B D+ cells exposed to G-CSF, while, in contrast, the cytokine supported growth in Ba/F3 cells transfected with the expression plasmid in the absence of IL-3. Western blotting, immunoprecipitation and cell surface labeling with Na[125I] followed by immunoprecipitation with an anti-G-CSFR antibody revealed different patterns of detected proteins in these two cell lines, implying that different forms of the G-CSFR were generated which produced distinct biological responses and/or that different cell-type specific signal transducers were involved in the production of cellular differentiation or growth. |
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Authors:
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J Li; A C Sartorelli |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Oncology research Volume: 7 ISSN: 0965-0407 ISO Abbreviation: Oncol. Res. Publication Date: 1995 |
Date Detail:
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Created Date: 1996-09-06 Completed Date: 1996-09-06 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 9208097 Medline TA: Oncol Res Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 591-601 Citation Subset: IM |
Affiliation:
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Department of Pharmacology, Yale University School of Medicine, New Haven, CT 06520, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Differentiation Cell Division Granulocyte Colony-Stimulating Factor / pharmacology Leukemia, Myeloid / pathology Mice Molecular Weight Peroxidase / genetics Receptors, Granulocyte Colony-Stimulating Factor / analysis, genetics, physiology* Transfection Tumor Cells, Cultured |
| Grant Support | |
ID/Acronym/Agency:
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CA-02817/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Receptors, Granulocyte Colony-Stimulating Factor; 143011-72-7/Granulocyte Colony-Stimulating Factor; EC 1.11.1.7/Peroxidase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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