Document Detail

Granulocyte colony-stimulating factor (G-CSF)-mediated signaling regulates type IV collagenase activity in head and neck cancer cells.
MedLine Citation:
PMID:  11391619     Owner:  NLM     Status:  MEDLINE    
Granulocyte colony-stimulating factor (G-CSF), a hematopoietic cytokine, regulates the proliferation and differentiation of granulocytic progenitor cells and functionally activated mature neutrophils. G-CSF also affects nonhematopoietic tumor cells through its binding to the specific receptor (G-CSFR) on the cells. The type IV collagenase [matrix metalloproteinase 2 (MMP-2)] is known to play a main role in the process of invasion and metastasis, but its regulation, for example, in expression or in activation, is not clearly understood. In this study, we investigated the role of G-CSF in the regulation of tumor cell invasion and the synthesis of MMP-2. G-CSFs producing the head and neck carcinoma cell line T3M-1 cells with metastatic ability and no G-CSF receptor (G-CSFR) expression were transfected with G-CSFR expression vector. In vitro treatment of G-CSFR-transfectant T3M-1 cells with recombinant G-CSF (rG-CSF) significantly augmented their invasive potential in a reconstituted basement membrane (Matrigel) system compared with that of parental cells. Moreover, MMP-2 activity of G-CSFR-transfectant T3M-1 cells was enhanced by the stimulation with rG-CSF, as assessed by gelatin zymography. These results identify G-CSF as a regulator of MMP-2 and cellular invasion.
C Sugimoto; S Fujieda; H Sunaga; I Noda; N Tanaka; Y Kimura; H Saito; S Matsukawa
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  International journal of cancer. Journal international du cancer     Volume:  93     ISSN:  0020-7136     ISO Abbreviation:  Int. J. Cancer     Publication Date:  2001 Jul 
Date Detail:
Created Date:  2001-06-06     Completed Date:  2001-06-28     Revised Date:  2007-07-24    
Medline Journal Info:
Nlm Unique ID:  0042124     Medline TA:  Int J Cancer     Country:  United States    
Other Details:
Languages:  eng     Pagination:  42-6     Citation Subset:  IM    
Copyright Information:
Copyright 2001 Wiley-Liss, Inc.
Department of Otorhinolaryngology, Fukui Medical University, Fukui, Japan.
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MeSH Terms
Carcinoma, Squamous Cell / pathology
Gene Expression Regulation, Enzymologic / drug effects
Gene Expression Regulation, Neoplastic / drug effects
Granulocyte Colony Stimulating Factor, Recombinant / pharmacology*
Head and Neck Neoplasms
Matrix Metalloproteinase 2 / genetics*
Mouth Neoplasms / pathology
Neoplasm Invasiveness
RNA, Messenger / genetics
Receptors, Granulocyte Colony-Stimulating Factor / drug effects,  genetics,  physiology*
Recombinant Proteins / drug effects,  metabolism
Reverse Transcriptase Polymerase Chain Reaction
Signal Transduction / drug effects,  physiology*
Transcription, Genetic / drug effects
Tumor Cells, Cultured / pathology
Reg. No./Substance:
0/Granulocyte Colony Stimulating Factor, Recombinant; 0/RNA, Messenger; 0/Receptors, Granulocyte Colony-Stimulating Factor; 0/Recombinant Proteins; EC Metalloproteinase 2

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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