Document Detail


Gram-staining characterisation of activated sludge filamentous bacteria by automated colour analysis.
MedLine Citation:
PMID:  15672225     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
An automated image analysis method has been developed for the monitoring of the Gram-staining characteristics of filamentous bacteria in activated sludge. The binary method of pixel classification agreed with manual estimation (level of correlation of 0.9 for Gram-positive bacteria). Its robustness has been assessed by repeatability tests. Population shifts in terms of Gram-staining characteristics have been monitored in laboratory-scale experiments with two feeding schedules using this technique.
Authors:
Denis Pandolfi; Marie-Noëlle Pons
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Publication Detail:
Type:  Comparative Study; Evaluation Studies; Journal Article; Validation Studies    
Journal Detail:
Title:  Biotechnology letters     Volume:  26     ISSN:  0141-5492     ISO Abbreviation:  Biotechnol. Lett.     Publication Date:  2004 Dec 
Date Detail:
Created Date:  2005-01-26     Completed Date:  2005-07-13     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8008051     Medline TA:  Biotechnol Lett     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  1841-6     Citation Subset:  IM    
Affiliation:
Laboratoire des Sciences du Génie Chimique, CNRS-ENSIC-INPL, 1, rue Grandville, BP 451, F-54001 Nancy cedex, France.
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MeSH Terms
Descriptor/Qualifier:
Colony Count, Microbial / methods
Colorimetry / methods*
Fuzzy Logic
Gentian Violet*
Gram-Negative Bacteria / cytology*,  isolation & purification*
Gram-Positive Bacteria / cytology*,  isolation & purification*
Image Interpretation, Computer-Assisted / methods*
Pattern Recognition, Automated / methods
Phenazines*
Pilot Projects
Reproducibility of Results
Sensitivity and Specificity
Sewage / microbiology*
Chemical
Reg. No./Substance:
0/Gram's stain; 0/Phenazines; 0/Sewage; 548-62-9/Gentian Violet

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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