Document Detail

Gram-negative pathogen Klebsiella oxytoca is associated with spontaneous chronic otitis media in Toll-like receptor 4-deficient C3H/HeJ mice.
MedLine Citation:
PMID:  17851949     Owner:  NLM     Status:  MEDLINE    
CONCLUSION: This report confirms the presence of gram-negative Klebsiella bacteria in the middle ear of the C3H/HeJ mouse by culture, polymerase chain reaction (PCR), and electron microscopy. Identification of the bacterial pathogen supports the C3H/HeJ mouse as an excellent model for spontaneous chronic otitis media and its effects on the middle and inner ear.
OBJECTIVES: The C3H/HeJ mouse has a single amino acid substitution in its Toll-like receptor 4, making it insensitive to endotoxin and suppressing initiation of the innate immune system. This study explored the bacteriology of the resultant middle ear infection by culture, PCR, histology, and electron microscopy.
MATERIALS AND METHODS: Twelve-month-old C3H/ HeJ mice were screened positive for spontaneous otitis media. Tympanocentesis and blood cultures of mice were carried out under sedation. Middle ear aspirate material and blood samples were then sent for culture and PCR. Mice were then sacrificed for bright-field and electron microscopy analysis.
RESULTS: All tympanocentesis and blood specimens grew gram-negative Klebsiella oxytoca, which was confirmed by PCR. Histopathology confirmed an intense inflammatory reaction and gram-negative bacteria in the middle and inner ears. Electron microscopy of the middle ears revealed abundant rod-shaped Klebsiella bacteria, both free and being engulfed by neutrophils.
Carol J MacArthur; De-Ann M Pillers; Jiaqing Pang; Jacqueline M Degagne; J Beth Kempton; Dennis R Trune
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Acta oto-laryngologica     Volume:  128     ISSN:  0001-6489     ISO Abbreviation:  Acta Otolaryngol.     Publication Date:  2008 Feb 
Date Detail:
Created Date:  2008-07-24     Completed Date:  2008-08-19     Revised Date:  2014-05-08    
Medline Journal Info:
Nlm Unique ID:  0370354     Medline TA:  Acta Otolaryngol     Country:  Norway    
Other Details:
Languages:  eng     Pagination:  132-8     Citation Subset:  IM    
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MeSH Terms
Bacteriological Techniques
Chronic Disease
Disease Models, Animal*
Ear, Middle / pathology
Immune Tolerance / genetics,  immunology
Immunity, Innate / genetics,  immunology
Klebsiella Infections / genetics*,  immunology,  pathology
Klebsiella oxytoca* / growth & development
Mice, Inbred C3H
Microscopy, Electron
Otitis Media / genetics*,  immunology,  pathology
Polymerase Chain Reaction
Toll-Like Receptor 4 / genetics*
Grant Support
Reg. No./Substance:
0/Tlr4 protein, mouse; 0/Toll-Like Receptor 4

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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