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Gram-negative bacterial LPS induced poor uterine receptivity and implantation failure in mouse: alterations in IL-1beta expression in the preimplantation embryo and uterine horns.
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MedLine Citation:
PMID:  16126496     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Genito-urinary tract or systemic infections of the gram-negative bacteria in pregnant women, causes abortions, preterm labor, and several other perinatal complications. LPS is the most potent antigenic component of the gram-negative bacterial cell wall and is known to modulate the expression of various proinflammatory cytokines. Here we investigate the role of the soluble form of IL-1 i.e., IL-1beta in the 'minimum dose' of LPS induced pregnancy loss in mice. Uterine cross-sections on each day of the preimplantation period of pregnancy were examined histopathologically for finding out LPS induced changes in the uterine preparation for embryo implantation. The expression of IL-1beta in the various stages of the preimplantation period of pregnancy was studied by RT-PCR in the embryos and the uterine horns of the LPS treated and normal pregnant mice. We found that LPS significantly alters the proliferation of the glandular epithelium, luminal epithelium and stroma during the preimplantation period. We also found large infiltration of macrophages into the uterine horns of the LPS treated animals. The level and pattern of IL-1beta expression in the preimplantation embryos and uterine horns were also altered in LPS treated animals. These observations indicate that LPS can alter the uterine preparation for blastocyst implantation, which could be due to the change in the IL-1beta expression in the uterine horns. However, a change in the expression pattern of IL-1beta in the preimplantation embryos underlines the significance of this molecule in LPS induced pregnancy loss or implantation failure in mouse.
Authors:
Kaushik Deb; Madan Mohan Chaturvedi; Yogesh Kumar Jaiswal
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Infectious diseases in obstetrics and gynecology     Volume:  13     ISSN:  1064-7449     ISO Abbreviation:  Infect Dis Obstet Gynecol     Publication Date:  2005 Sep 
Date Detail:
Created Date:  2005-08-29     Completed Date:  2006-01-06     Revised Date:  2008-11-20    
Medline Journal Info:
Nlm Unique ID:  9318481     Medline TA:  Infect Dis Obstet Gynecol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  125-33     Citation Subset:  IM    
Affiliation:
Molecular Biology & Reproductive Immunology Laboratory, School of Studies in Biochemistry, Jiwaji University, Gwalior, India. debk@missouri.edu
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MeSH Terms
Descriptor/Qualifier:
Animals
Blastocyst / chemistry*
Embryo Implantation / drug effects*
Female
Gene Expression / drug effects
Gestational Age
Gram-Negative Bacteria*
Interleukin-1 / genetics*
Lipopolysaccharides / administration & dosage,  pharmacology*
Mice
Pregnancy
RNA, Messenger / analysis
Reverse Transcriptase Polymerase Chain Reaction
Superovulation
Uterus / chemistry,  drug effects*,  physiology
Chemical
Reg. No./Substance:
0/Interleukin-1; 0/Lipopolysaccharides; 0/RNA, Messenger

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Full Text
Journal Information
Journal ID (nlm-ta): Infect Dis Obstet Gynecol
ISSN: 1064-7449
ISSN: 1098-0997
Article Information
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Print publication date: Month: 9 Year: 2005
Volume: 13 Issue: 3
First Page: 125 Last Page: 133
ID: 1784569
Publisher Item Identifier: V7870VN156219735
DOI: 10.1080/10647440500147885
PubMed Id: 16126496

Gram-negative bacterial LPS induced poor uterine receptivity and implantation failure in mouse: alterations in IL-1beta expression in the preimplantation embryo and uterine horns.
Kaushik Deb Email: debk@missouri.edu
Madan Mohan Chaturvedi
Yogesh Kumar Jaiswal
Molecular Biology & Reproductive Immunology Laboratory, School of Studies in Biochemistry, Jiwaji University, Gwalior, India.


Article Categories:
  • Research Article


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