| Graft derived cells with double nuclei in the penumbral region of experimental brain trauma. | |
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MedLine Citation:
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PMID: 16377084 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Recent in vitro studies showed that stem cells might fuse with mature cells or each other; however, there is no in vivo evidence for this phenomenon in the cerebral cortex. Our goal was to find evidence for cell fusion in a model of traumatic brain injury followed by grafting of embryonic cortical cells. Cold lesion protocol was applied to induce lesion of the motor cortex in adult male rats. Six days later we grafted a suspension of freshly isolated rat brain cortical cells of early embryonic stage (E14) into the penumbra area of the lesion. The grafted cell nuclei were labelled with bromodeoxyuridine (BrDU). Six days after transplantation 4,328 BrDU positive cells were observed in nine animals. 89.5% of these cells had cytoplasmic staining probably representing dead or phagocyted grafted cells. Ten percent of surviving BrDU positive cells had only one BrDU positive nucleus and negative cytoplasm, while 0.5% had two distinct nuclei, one was unlabelled and one was BrDU positive. These cells were similar in appearance and size to the astrocytes in the vicinity and expressed the astocyte specific glial fibrillaly acidic protein. Thus, these cells showed a possible sign of cell fusion in the penumbral region of the injured brain. |
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Authors:
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Eszter M Horváth; Zsombor Lacza; Attila Csordás; Csaba Szabó; Márk Kollai; David W Busija |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2005-12-27 |
Journal Detail:
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Title: Neuroscience letters Volume: 396 ISSN: 0304-3940 ISO Abbreviation: Neurosci. Lett. Publication Date: 2006 Apr |
Date Detail:
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Created Date: 2006-03-06 Completed Date: 2006-05-25 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 7600130 Medline TA: Neurosci Lett Country: Ireland |
Other Details:
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Languages: eng Pagination: 182-6 Citation Subset: IM |
Affiliation:
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Department of Physiology and Pharmacology, Wake Forest University Health Sciences, Winston-Salem, NC, USA. horvath.eszter.maria@1997.aok.usn.hu |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Brain Injuries* / pathology, physiopathology, surgery Brain Tissue Transplantation / methods* Bromodeoxyuridine / metabolism Cell Count / methods Cell Division / physiology* Cell Nucleus* / metabolism Cell Transplantation / methods* Cerebral Cortex / cytology Disease Models, Animal Embryo, Mammalian Female Glial Fibrillary Acidic Protein / metabolism Immunohistochemistry / methods Indoles / diagnostic use Intermediate Filament Proteins / metabolism Male Nerve Tissue Proteins / metabolism Neurofilament Proteins / metabolism Pregnancy Rats |
| Grant Support | |
ID/Acronym/Agency:
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HL30260/HL/NHLBI NIH HHS; HL46558/HL/NHLBI NIH HHS; HL50587/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Glial Fibrillary Acidic Protein; 0/Indoles; 0/Intermediate Filament Proteins; 0/Nerve Tissue Proteins; 0/Neurofilament Proteins; 0/nestin; 47165-04-8/DAPI; 59-14-3/Bromodeoxyuridine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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