Document Detail


Graft cytokine mRNA activity in rat single lung transplants by reverse transcription-polymerase chain reaction: effect of cyclosporine.
MedLine Citation:
PMID:  1457427     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Intragraft levels of cytokine mRNA were studied in an orthotopic rat left lung transplant model. Three groups of rats were compared at 7 days after transplantation. Isogeneic (Lewis to Lewis), allogeneic (Brown-Norway to Lewis) untreated, and cyclosporine-treated (25 mg/kg/day, intramuscularly) allogeneic animals underwent analysis of cytokine mRNA isolated from total RNA in freshly excised grafts. Reverse transcription-polymerase chain reaction amplification of interleukin (IL)-2, IL-4, and actin (control) mRNA was performed with custom-synthesized oligonucleotide amplimers targeted to known sequences of rat IL-2 and IL-4 cDNA. Semiquantitative analysis was performed by radioanalytic scanning of gel preparations. Sample specimens from the retrieved grafts were also graded histologically for rejection on a five-point scale. Rejection was most severe in the untreated allografts (p < 0.003). IL-2 mRNA was significantly greater in the untreated allografts when compared with isografts (p < 0.05) and cyclosporine-treated allografts (p < 0.05). No significant differences in IL-4 mRNA between groups were observed. We conclude that semiquantitative analysis of cytokine mRNA by reverse transcription-polymerase chain reaction is a useful and sensitive method for the study of acute rejection in lung grafts and that this technique may become an important tool in future studies of cytokine-mediated responses in cyclosporine-treated allografts.
Authors:
N A Francalancia; S C Wang; N L Thai; R Aeba; R L Simmons; S A Yousem; R L Hardesty; B P Griffith
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  The Journal of heart and lung transplantation : the official publication of the International Society for Heart Transplantation     Volume:  11     ISSN:  1053-2498     ISO Abbreviation:  J. Heart Lung Transplant.     Publication Date:    1992 Nov-Dec
Date Detail:
Created Date:  1993-01-12     Completed Date:  1993-01-12     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9102703     Medline TA:  J Heart Lung Transplant     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1041-5     Citation Subset:  IM    
Affiliation:
Department of Surgery, University of Pittsburgh, Pa.
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MeSH Terms
Descriptor/Qualifier:
Actins / genetics*
Animals
Cyclosporine / therapeutic use*
Graft Rejection / diagnosis,  genetics*
Immunosuppression
Interleukin-2 / genetics*
Interleukin-4 / genetics*
Lung Transplantation / immunology*
Male
Polymerase Chain Reaction
RNA, Messenger / metabolism*
Rats
Rats, Inbred BN
Rats, Inbred Lew
Time Factors
Transcription, Genetic
Chemical
Reg. No./Substance:
0/Actins; 0/Interleukin-2; 0/RNA, Messenger; 207137-56-2/Interleukin-4; 59865-13-3/Cyclosporine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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