| Graft cytokine mRNA activity in rat single lung transplants by reverse transcription-polymerase chain reaction: effect of cyclosporine. | |
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MedLine Citation:
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PMID: 1457427 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Intragraft levels of cytokine mRNA were studied in an orthotopic rat left lung transplant model. Three groups of rats were compared at 7 days after transplantation. Isogeneic (Lewis to Lewis), allogeneic (Brown-Norway to Lewis) untreated, and cyclosporine-treated (25 mg/kg/day, intramuscularly) allogeneic animals underwent analysis of cytokine mRNA isolated from total RNA in freshly excised grafts. Reverse transcription-polymerase chain reaction amplification of interleukin (IL)-2, IL-4, and actin (control) mRNA was performed with custom-synthesized oligonucleotide amplimers targeted to known sequences of rat IL-2 and IL-4 cDNA. Semiquantitative analysis was performed by radioanalytic scanning of gel preparations. Sample specimens from the retrieved grafts were also graded histologically for rejection on a five-point scale. Rejection was most severe in the untreated allografts (p < 0.003). IL-2 mRNA was significantly greater in the untreated allografts when compared with isografts (p < 0.05) and cyclosporine-treated allografts (p < 0.05). No significant differences in IL-4 mRNA between groups were observed. We conclude that semiquantitative analysis of cytokine mRNA by reverse transcription-polymerase chain reaction is a useful and sensitive method for the study of acute rejection in lung grafts and that this technique may become an important tool in future studies of cytokine-mediated responses in cyclosporine-treated allografts. |
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Authors:
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N A Francalancia; S C Wang; N L Thai; R Aeba; R L Simmons; S A Yousem; R L Hardesty; B P Griffith |
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Publication Detail:
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Type: Comparative Study; Journal Article |
Journal Detail:
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Title: The Journal of heart and lung transplantation : the official publication of the International Society for Heart Transplantation Volume: 11 ISSN: 1053-2498 ISO Abbreviation: J. Heart Lung Transplant. Publication Date: 1992 Nov-Dec |
Date Detail:
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Created Date: 1993-01-12 Completed Date: 1993-01-12 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9102703 Medline TA: J Heart Lung Transplant Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1041-5 Citation Subset: IM |
Affiliation:
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Department of Surgery, University of Pittsburgh, Pa. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Actins
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genetics* Animals Cyclosporine / therapeutic use* Graft Rejection / diagnosis, genetics* Immunosuppression Interleukin-2 / genetics* Interleukin-4 / genetics* Lung Transplantation / immunology* Male Polymerase Chain Reaction RNA, Messenger / metabolism* Rats Rats, Inbred BN Rats, Inbred Lew Time Factors Transcription, Genetic |
| Chemical | |
Reg. No./Substance:
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0/Actins; 0/Interleukin-2; 0/RNA, Messenger; 207137-56-2/Interleukin-4; 59865-13-3/Cyclosporine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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