| Graded and lamina-specific distributions of ligands of EphB receptor tyrosine kinases in the developing retinotectal system. | |
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MedLine Citation:
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PMID: 9356168 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Molecular gradients have been postulated to control the topographic mapping of retinal axons in their central targets. Based initially on their expression patterns, and more recently on functional studies, members of the EphA subfamily of receptor tyrosine kinases and their ephrin-A ligands have been implicated in the guidance of retinal axons along the anterior-posterior axis of the chick optic tectum. The report that a receptor of the EphB subfamily, EphB2/Cek5/Nuk/Sek3, is expressed in a high ventral to low dorsal gradient in the developing chick retina and is present on ganglion cell axons suggests that it may be involved in the mapping of retinal axons along the corresponding dorsal-ventral axis of the tectum. To address this issue, we have determined the expression and distribution of ephrin-B1/LERK-2/Cek5-L and ephrin-B2/LERK-5/Htk-L/ELF-2, ligands for EphB2, in the developing chick retinotectal system using riboprobes, immunocytochemistry, and receptor affinity probes. Both ephrin-B1 and ephrin-B2 transcripts are expressed in a high dorsal to low ventral gradient in the developing retina, complementary to the distribution of EphB2. Ephrin-B1 and ephrin-B2 proteins are predominantly found in the developing plexiform layers, suggesting a role in the development of intraretinal connections. Neither protein is detected on ganglion cell axons. In tectum, ephrin-B1 transcripts are expressed in a high dorsal to low ventral gradient in the neuroepithelium and the protein is present along the processes of radial glia and is concentrated at their endfeet in the stratum opticum, at the time retinal axons are growing through it. This distribution of ephrin-B1 suggests that it influences retinal axon mapping along the dorsal-ventral tectal axis and may also be involved in intratectal development. In contrast, ephrin-B2 transcripts and protein are localized to the deeper retinorecipient laminae in the tectum at the time retinal axons begin to arborize in them, suggesting that this ligand may influence the laminar patterning of retinal axon terminations. |
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Authors:
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J E Braisted; T McLaughlin; H U Wang; G C Friedman; D J Anderson; D D O'leary |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Developmental biology Volume: 191 ISSN: 0012-1606 ISO Abbreviation: Dev. Biol. Publication Date: 1997 Nov |
Date Detail:
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Created Date: 1997-12-05 Completed Date: 1997-12-05 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 0372762 Medline TA: Dev Biol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 14-28 Citation Subset: IM |
Copyright Information:
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Copyright 1997 Academic Press. |
Affiliation:
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Molecular Neurobiology Laboratory, The Salk Institute, 10010 North Torrey Pines Road, La Jolla, California 92037, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Axons / metabolism Body Patterning Chick Embryo DNA Primers Ephrin-B1 Gene Expression Regulation, Developmental Membrane Proteins / biosynthesis Polymerase Chain Reaction Receptor Protein-Tyrosine Kinases / biosynthesis* Receptor, EphB2 Retina / cytology, embryology*, metabolism Retinal Ganglion Cells / cytology, metabolism Superior Colliculi / embryology*, metabolism Transcription, Genetic |
| Grant Support | |
ID/Acronym/Agency:
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F32 EY06550/EY/NEI NIH HHS; F32 EY06769/EY/NEI NIH HHS; R01 EY07025/EY/NEI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/DNA Primers; 0/Ephrin-B1; 0/Membrane Proteins; EC 2.7.10.1/Receptor Protein-Tyrosine Kinases; EC 2.7.10.1/Receptor, EphB2 |
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