Document Detail

Grade-dependent effects on cell cycle progression and apoptosis in response to doxorubicin in human bladder cancer cell lines.
MedLine Citation:
PMID:  19082486     Owner:  NLM     Status:  MEDLINE    
Doxorubicin is an important component of combination therapy for muscle-invasive urinary bladder cancer. Treatment with this topoisomerase II poison is able to interfere with cell cycle progression and lead to cancer cell death. Using FACS analysis, Western immunoblotting and semi-quantitative RT-PCR, we studied the effects of doxorubicin on cell cycle progression and apoptosis, and also explored the possibility of using groups of genes as biomarkers of prognosis and/or response to doxorubicin treatment in human urinary bladder cancer cells. Doxorubicin induced dose-dependent G2/M and/or G1/S cell cycle arrest, followed by grade- and dose-dependent reduction in the amount of the cytosolic trimeric form of FasL, activation of Caspase-8, Caspase-9, Caspase-3, cleavage of PARP, Lamin A/C, Bcl-XL/S and interestingly Hsp90, and finally cell death. Data presented here also suggest the use of the expression patterns of Cyclin-E2, Cyclin-F, p63, p73, FasL, TRAIL, Tweak, Tweak-R, XAF-1, OPG and Bok genes for identification of the differentiation grade, and Cyclin-B2, GADD45A, p73, FasL, Bik, Bim, TRAIL, Fas, Tweak-R, XAF-1, Bcl-2, Survivin, OPG, DcR2 and Bcl-XL genes for the detection of response to doxorubicin in human bladder cancer cells.
Dimitrios J Stravopodis; Panagiotis K Karkoulis; Eumorphia G Konstantakou; Sophia Melachroinou; Antonis D Lampidonis; Dimitra Anastasiou; Stefanos Kachrilas; Niki Messini-Nikolaki; Issidora S Papassideri; Gerasimos Aravantinos; Lukas H Margaritis; Gerassimos E Voutsinas
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  International journal of oncology     Volume:  34     ISSN:  1019-6439     ISO Abbreviation:  Int. J. Oncol.     Publication Date:  2009 Jan 
Date Detail:
Created Date:  2008-12-16     Completed Date:  2009-02-24     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9306042     Medline TA:  Int J Oncol     Country:  Greece    
Other Details:
Languages:  eng     Pagination:  137-60     Citation Subset:  IM    
Department of Cell Biology and Biophysics, Faculty of Biology, University of Athens, Panepistimiopolis, Zografou, 15784 Athens, Greece.
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MeSH Terms
Antibiotics, Antineoplastic / pharmacology*
Apoptosis / drug effects*
Blotting, Western
Carcinoma, Transitional Cell / metabolism,  pathology*
Cell Cycle / drug effects*
Cell Cycle Proteins / metabolism
Cell Differentiation / drug effects
Cell Line, Tumor
Cell Proliferation / drug effects
Doxorubicin / pharmacology*
Flow Cytometry
Gene Expression Profiling
Neoplasm Staging
Oligonucleotide Array Sequence Analysis
RNA, Messenger / genetics,  metabolism
Reverse Transcriptase Polymerase Chain Reaction
Tumor Markers, Biological
Urinary Bladder Neoplasms / metabolism,  pathology*
Reg. No./Substance:
0/Antibiotics, Antineoplastic; 0/Cell Cycle Proteins; 0/RNA, Messenger; 0/Tumor Markers, Biological; 23214-92-8/Doxorubicin

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