Document Detail


Gossypol isomers bind specifically to blood plasma proteins and spermatozoa of rainbow trout fed diets containing cottonseed meal.
MedLine Citation:
PMID:  11342251     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We investigated the role of gossypol isomers binding to blood plasma, seminal plasma and spermatozoa to elucidate gossypol anti-fertility action in the teleost fish, rainbow trout (Oncorhynchus mykiss). Growth and hematological indicators of males were depressed when fish meal protein in diets was completely replaced with cottonseed meal. The cottonseed meal contained equal proportions of (-) (47.8+/-1.6%) and (+) gossypol isomers. Concentrations of spermatozoa were decreased with increasing proportions of gossypol in diets (from 0.22% to 0.95%); however, sperm motility and fertilizing ability were not affected. In contrast to mammals, steroid hormone concentrations were not suppressed in fish given diets with gradual increase of gossypol level. Gossypol concentrations were 100-fold higher in blood plasma than in seminal plasma, confirming a barrier in gossypol transfer between the general circulation and the testis. Spermatozoa accumulated predominantly (+) enantiomer (65-75%) with decreasing proportions as dietary gossypol concentrations increased. Spermatozoa bound most of the gossypol contained in the semen; however, this did not result in impairment of the sperm motility apparatus. Teleost fish sperm rely on ATP stores that accumulate during maturation as a source of energy during activation. In addition, the duration of sperm movement is short in these fish. As such, we hypothesize that the major action of gossypol on mammalian sperm, which is uncoupling of oxidative phosphorylation, does not impair the energy supply required for flagellar beating in fish spermatozoa.
Authors:
K Dabrowski; K J Lee; J Rinchard; A Ciereszko; J H Blom; J S Ottobre
Publication Detail:
Type:  Comparative Study; In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  1525     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  2001 Feb 
Date Detail:
Created Date:  2001-05-08     Completed Date:  2001-05-21     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  37-42     Citation Subset:  IM    
Affiliation:
School of Natural Resources and Piketon Research and Extension Center, The Ohio State University, Columbus 43210-1085, USA. dabrowski.1@osu.edu
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MeSH Terms
Descriptor/Qualifier:
Animals
Blood Proteins / metabolism*
Cottonseed Oil / adverse effects
Diet / adverse effects
Fertility / drug effects
Gossypol / chemistry,  metabolism*,  toxicity
Male
Mammals
Oncorhynchus mykiss / blood,  metabolism*
Protein Binding
Semen / metabolism
Sperm Motility / drug effects
Spermatozoa / metabolism*
Stereoisomerism
Chemical
Reg. No./Substance:
0/Blood Proteins; 0/Cottonseed Oil; 303-45-7/Gossypol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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