Document Detail


Gordonan, an acidic polysaccharide with cell aggregation-inducing activity in insect BM-N4 cells, produced by Gordonia sp.
MedLine Citation:
PMID:  11193406     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
An acidic polysaccharide, termed gordonan, was isolated from the culture medium of Gordonia sp. as an inducer of cell aggregation in an insect cell line, BM-N4. Gordonan had an average molecular weight of 5 x 10(6) and its structure was identified as -->3)-4-O-(1-carboxyethyl)-beta-D-Manp-(1-->4)-beta-D-GlcAp-(1-->4)-beta-D-Glcp-(1--> mainly by acid hydrolysis experiments and NMR analysis. It induces cell aggregation at the concentration of 4 microg/ml. A partially hydrolyzed polysaccharide derived from gordonan with a molecular weight of 5 x 10(5) showed weak activity, while any fragment molecules with lower molecular weights prepared from gordonan showed no activity.
Authors:
T Kondo; D Yamamoto; A Yokota; A Suzuki; H Nagasawa; S Sakuda
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Bioscience, biotechnology, and biochemistry     Volume:  64     ISSN:  0916-8451     ISO Abbreviation:  Biosci. Biotechnol. Biochem.     Publication Date:  2000 Nov 
Date Detail:
Created Date:  2001-01-19     Completed Date:  2001-03-08     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9205717     Medline TA:  Biosci Biotechnol Biochem     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  2388-94     Citation Subset:  IM    
Affiliation:
Department of Applied Biological Chemistry, The University of Tokyo, Japan.
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MeSH Terms
Descriptor/Qualifier:
Actinomycetales / chemistry*
Animals
Cell Aggregation / drug effects
Cells, Cultured
Fermentation
Insects
Magnetic Resonance Spectroscopy
Polysaccharides / chemistry,  isolation & purification*,  pharmacology
Polysaccharides, Bacterial / chemistry,  isolation & purification*,  pharmacology
Chemical
Reg. No./Substance:
0/Polysaccharides; 0/Polysaccharides, Bacterial; 0/gordonan

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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