Document Detail


Goosecoid suppresses cell growth and enhances neuronal differentiation of PC12 cells.
MedLine Citation:
PMID:  10893186     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In all vertebrate species, the homeobox gene goosecoid serves as a marker of the Spemann organizer tissue. One function of the organizer is the induction of neural tissue. To investigate the role of goosecoid in neuronal differentiation of mammalian cells, we have introduced goosecoid into PC12 cells. Expression of goosecoid resulted in reduced cell proliferation and enhanced neurite outgrowth in response to NGF. Expression of goosecoid led to a decrease in the percentage of S-phase cells and to upregulation of the expression of the neuron-specific markers MAP-1b and neurofilament-L. Analysis of goosecoid mutants revealed that these effects were independent of either DNA binding or homodimerization of Goosecoid. Coexpression of the N-terminal portion of the ets transcription factor PU.1, a protein that can bind to Goosecoid, repressed neurite outgrowth and rescued the proliferation of PC12 cultures. In contrast, expression of the bHLH transcription factor HES-1 repressed goosecoid-mediated neurite outgrowth without changing the proportion of S-phase cells. These results suggest that goosecoid is involved in neuronal differentiation in two ways, by slowing the cell cycle and stimulating neurite outgrowth, and that these two events are separately regulated.
Authors:
K Sawada; Y Konishi; M Tominaga; Y Watanabe; J Hirano; S Inoue; R Kageyama; M Blum; A Tominaga
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of cell science     Volume:  113 ( Pt 15)     ISSN:  0021-9533     ISO Abbreviation:  J. Cell. Sci.     Publication Date:  2000 Aug 
Date Detail:
Created Date:  2000-09-21     Completed Date:  2000-09-21     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0052457     Medline TA:  J Cell Sci     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  2705-13     Citation Subset:  IM    
Affiliation:
Department of Neuropsychiatry, Kochi Medical School, Kohasu, Okoh-cho, Nankoku City, Kochi 783-8505, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Basic Helix-Loop-Helix Transcription Factors
Biological Markers
Cell Differentiation / drug effects,  physiology
Cell Division / drug effects,  physiology
Gene Expression / drug effects,  physiology
Goosecoid Protein
Homeodomain Proteins / genetics*
Microtubule-Associated Proteins / analysis
Mutagenesis / physiology
Nerve Growth Factor / pharmacology
Neurites / drug effects,  physiology*
Neurofilament Proteins / genetics
Neurons / chemistry,  physiology*,  ultrastructure*
PC12 Cells
Proto-Oncogene Proteins / genetics
Rats
Repressor Proteins / genetics
S Phase / drug effects,  physiology
Trans-Activators / genetics
Transcription Factors*
Transfection
Chemical
Reg. No./Substance:
0/Basic Helix-Loop-Helix Transcription Factors; 0/Biological Markers; 0/Goosecoid Protein; 0/Gsc protein, rat; 0/Hes1 protein, rat; 0/Homeodomain Proteins; 0/Microtubule-Associated Proteins; 0/Neurofilament Proteins; 0/Proto-Oncogene Proteins; 0/Repressor Proteins; 0/Trans-Activators; 0/Transcription Factors; 0/microtubule-associated protein 1B; 0/neurofilament protein L; 0/proto-oncogene protein Spi-1; 9061-61-4/Nerve Growth Factor

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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