| Gonadotrophin-stimulated cyclic AMP production by granulosa cells from Booroola x Romney ewes with and without a fecundity gene. | |
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MedLine Citation:
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PMID: 2993605 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The influence of follicular size and health on FSH and LH stimulation of cAMP production by granulosa cells in vitro was studied in cells from Booroola X Romney ewes, with (F+) and without (++) a fecundity gene. The granulosa cells were obtained 0-48 h after the initiation of luteolysis on Day 10 of the oestrous cycle by cloprostenol. The highest mean amounts of cAMP produced by granulosa cells challenged with FSH or LH were not significantly different between the genotypes. However, they were achieved using granulosa cells from follicles greater than 3-4 mm in diameter in F+ ewes but from follicles greater than 4 mm in diameter in ++ ewes. Follicles may thus attain ovulatory maturity at a smaller diameter in F+ ewes than in ++ ewes. Granulosa cells from most atretic follicles gave a poor cAMP response to FSH or LH, compared to cells from non-atretic follicles. Granulosa cell responsiveness to FSH was independent of the time the cells were recovered after cloprostenol treatment in F+ ewes, but not in ++ ewes. Cellular responsiveness to LH was independent of time for sheep of both genotypes. There was a significant positive relationship for sheep of both genotypes between the level of aromatase activity in granulosa cells and cellular responsiveness to FSH and LH. |
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Authors:
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K M Henderson; L E Kieboom; K P McNatty; S Lun; D Heath |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of reproduction and fertility Volume: 75 ISSN: 0022-4251 ISO Abbreviation: J. Reprod. Fertil. Publication Date: 1985 Sep |
Date Detail:
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Created Date: 1985-10-24 Completed Date: 1985-10-24 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0376367 Medline TA: J Reprod Fertil Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 111-20 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Aromatase / metabolism Cloprostenol / pharmacology Cyclic AMP / biosynthesis* Female Fertility* Follicle Stimulating Hormone / pharmacology Genotype* Gonadotropins / pharmacology* Granulosa Cells / drug effects*, enzymology Luteinizing Hormone / pharmacology Ovarian Follicle / anatomy & histology Ovulation Sheep |
| Chemical | |
Reg. No./Substance:
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0/Gonadotropins; 40665-92-7/Cloprostenol; 60-92-4/Cyclic AMP; 9002-67-9/Luteinizing Hormone; 9002-68-0/Follicle Stimulating Hormone; EC 1.14.14.1/Aromatase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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