Document Detail


The Golgi protein GM130 regulates centrosome morphology and function.
MedLine Citation:
PMID:  18045989     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The Golgi apparatus (GA) of mammalian cells is positioned in the vicinity of the centrosome, the major microtubule organizing center of the cell. The significance of this physical proximity for organelle function and cell cycle progression is only beginning to being understood. We have identified a novel function for the GA protein, GM130, in the regulation of centrosome morphology, position and function during interphase. RNA interference-mediated depletion of GM130 from five human cell lines revealed abnormal interphase centrosomes that were mispositioned and defective with respect to microtubule organization and cell migration. When GM130-depleted cells entered mitosis, they formed multipolar spindles, arrested in metaphase, and died. We also detected aberrant centrosomes during interphase and multipolar spindles during mitosis in ldlG cells, which do not contain detectable GM130. Although GA proteins have been described to regulate mitotic centrosomes and spindle formation, this is the first report of a role for a GA protein in the regulation of centrosomes during interphase.
Authors:
Andrew Kodani; Christine Sütterlin
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-11-28
Journal Detail:
Title:  Molecular biology of the cell     Volume:  19     ISSN:  1939-4586     ISO Abbreviation:  Mol. Biol. Cell     Publication Date:  2008 Feb 
Date Detail:
Created Date:  2008-02-05     Completed Date:  2008-11-10     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  9201390     Medline TA:  Mol Biol Cell     Country:  United States    
Other Details:
Languages:  eng     Pagination:  745-53     Citation Subset:  IM    
Affiliation:
Developmental and Cell Biology, University of California, Irvine, Irvine, CA 92697-2300, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Autoantigens / genetics,  metabolism*
Base Sequence
CHO Cells
Cell Line, Tumor
Cell Movement
Cell Proliferation
Centrosome / metabolism*,  pathology
Cricetinae
Cricetulus
G2 Phase
Golgi Apparatus / metabolism*
Humans
Membrane Proteins / deficiency,  genetics,  metabolism*
Microtubules / metabolism
Mitosis
Mitotic Spindle Apparatus / metabolism,  pathology
Molecular Sequence Data
Grant Support
ID/Acronym/Agency:
//Howard Hughes Medical Institute
Chemical
Reg. No./Substance:
0/Autoantigens; 0/GORASP1 protein, human; 0/Golgin subfamily A member 2; 0/Membrane Proteins
Comments/Corrections

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