Document Detail


Golgi phosphoprotein 4 (GPP130) is a sensitive and selective cellular target of manganese exposure.
MedLine Citation:
PMID:  23280773     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Chronic elevated exposure to manganese (Mn) is associated with neurocognitive and fine motor deficits in children. However, relatively little is understood about cellular responses to Mn spanning the transition between physiologic to toxic levels of exposure. Here, we investigated the specificity, sensitivity, and time course of the Golgi Phosphoprotein 4 (GPP130) response to Mn exposure in AF5 GABAergic neuronal cells, and we determined the extent to which GPP130 degradation occurs in brain cells in vivo in rats subchronically exposed to Mn. Our results show that GPP130 degradation in AF5 cells was specific to Mn, and did not occur following exposure to cobalt, copper, iron, nickel, or zinc. GPP130 degradation occurred without measurable increases in intracellular Mn levels and at Mn exposures as low as 0.54 µM. GPP130 protein was detectable by immunofluorescence in only ∼15-30% of cells in striatal and cortical rat brain slices, and Mn-exposed animals exhibited a significant reduction in both the number of GPP130-positive cells, and the overall levels of GPP130 protein, demonstrating the in vivo relevance of this Mn-specific response within the primary target organ of Mn toxicity. These results provide insight into specific mechanism(s) of cellular Mn regulation and toxicity within the brain, including the selective susceptibility of cells to Mn cytotoxicity.
Authors:
Melisa Masuda; Michelle Braun-Sommargren; Dan Crooks; Donald R Smith
Related Documents :
22984433 - The hidden geometries of the arabidopsis thaliana epidermis.
23627463 - Concurrent overactivation of the cytosolic glutamine synthetase and the gaba shunt in t...
4605063 - Reuse of salmonella and shigella absorbing cells for preparing monospecific salmonella ...
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2013-02-08
Journal Detail:
Title:  Synapse (New York, N.Y.)     Volume:  67     ISSN:  1098-2396     ISO Abbreviation:  Synapse     Publication Date:  2013 May 
Date Detail:
Created Date:  2013-03-19     Completed Date:  2013-09-03     Revised Date:  2014-05-07    
Medline Journal Info:
Nlm Unique ID:  8806914     Medline TA:  Synapse     Country:  United States    
Other Details:
Languages:  eng     Pagination:  205-15     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 Wiley Periodicals, Inc.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Brain / metabolism
Cell Line
Female
GABAergic Neurons / metabolism
Manganese / pharmacology*
Proteolysis / drug effects*
Rats
Rats, Long-Evans
Vesicular Transport Proteins / genetics,  metabolism*
Grant Support
ID/Acronym/Agency:
R01 ES018990/ES/NIEHS NIH HHS; R01 ES019222/ES/NIEHS NIH HHS; R01ES018990/ES/NIEHS NIH HHS; R01ES019222/ES/NIEHS NIH HHS
Chemical
Reg. No./Substance:
0/Golgi phosphoprotein 4, rat; 0/Vesicular Transport Proteins; 42Z2K6ZL8P/Manganese
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Dorsal foot resurfacing using free anterolateral thigh (ALT) flap in children.
Next Document:  Xylanase supplementation improves the nutritive value of diets containing high levels of sorghum dis...