Document Detail

Golgi method without osmium tetroxide for the study of the central nervous system.
MedLine Citation:
PMID:  1284405     Owner:  NLM     Status:  MEDLINE    
A variant Golgi technique was developed that consisted of substituting osmium tetroxide with formaldehyde as the initial fixative in intracardiac perfusion, along with the addition of glacial acetic acid to the chromating fluid. This procedure avoids disposal of dangerous waste substances into the environment. Other advantages include 1) reduction of cost, danger to lab workers, and risk of disruption of the tissue slices during their handling by eliminating the osmium tetroxide, 2) clear tissue background, 3) greater quantity of impregnated neurons than in the classical procedure, with distinct morphological details easily identified even in gross sections and 4) reduction in processing time.
I González-Burgos; G Tapia-Arizmendi; A Feria-Velasco
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biotechnic & histochemistry : official publication of the Biological Stain Commission     Volume:  67     ISSN:  1052-0295     ISO Abbreviation:  Biotech Histochem     Publication Date:  1992 Sep 
Date Detail:
Created Date:  1993-05-26     Completed Date:  1993-05-26     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  9107378     Medline TA:  Biotech Histochem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  288-96     Citation Subset:  IM    
Laboratory of Morphology, Faculty of Biological Sciences, University of Guadalajara, Jalisco, Mexico.
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MeSH Terms
Brain / anatomy & histology
Central Nervous System / anatomy & histology*
Neuroglia / cytology
Neurons / cytology*
Osmium Tetroxide
Rats, Sprague-Dawley
Staining and Labeling / methods*
Tissue Embedding
Tissue Fixation
Reg. No./Substance:
20816-12-0/Osmium Tetroxide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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