Document Detail

The Golgi-localized Arabidopsis endomembrane protein12 contains both endoplasmic reticulum export and Golgi retention signals at its C terminus.
MedLine Citation:
PMID:  22570441     Owner:  NLM     Status:  MEDLINE    
Endomembrane proteins (EMPs), belonging to the evolutionarily conserved transmembrane nine superfamily in yeast and mammalian cells, are characterized by the presence of a large lumenal N terminus, nine transmembrane domains, and a short cytoplasmic tail. The Arabidopsis thaliana genome contains 12 EMP members (EMP1 to EMP12), but little is known about their protein subcellular localization and function. Here, we studied the subcellular localization and targeting mechanism of EMP12 in Arabidopsis and demonstrated that (1) both endogenous EMP12 (detected by EMP12 antibodies) and green fluorescent protein (GFP)-EMP12 fusion localized to the Golgi apparatus in transgenic Arabidopsis plants; (2) GFP fusion at the C terminus of EMP12 caused mislocalization of EMP12-GFP to reach post-Golgi compartments and vacuoles for degradation in Arabidopsis cells; (3) the EMP12 cytoplasmic tail contained dual sorting signals (i.e., an endoplasmic reticulum export motif and a Golgi retention signal that interacted with COPII and COPI subunits, respectively); and (4) the Golgi retention motif of EMP12 retained several post-Golgi membrane proteins within the Golgi apparatus in gain-of-function analysis. These sorting signals are highly conserved in all plant EMP isoforms and, thus, likely represent a general mechanism for EMP targeting in plant cells.
Caiji Gao; Christine K Y Yu; Song Qu; Melody Wan Yan San; Kwun Yee Li; Sze Wan Lo; Liwen Jiang
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-05-08
Journal Detail:
Title:  The Plant cell     Volume:  24     ISSN:  1532-298X     ISO Abbreviation:  Plant Cell     Publication Date:  2012 May 
Date Detail:
Created Date:  2012-06-28     Completed Date:  2012-10-25     Revised Date:  2013-06-25    
Medline Journal Info:
Nlm Unique ID:  9208688     Medline TA:  Plant Cell     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2086-104     Citation Subset:  IM    
School of Life Sciences, Centre for Cell and Developmental Biology, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China.
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MeSH Terms
Arabidopsis / metabolism*
Arabidopsis Proteins / metabolism*
Endoplasmic Reticulum / metabolism*
Golgi Apparatus / metabolism*
Models, Biological
Protein Transport / physiology
Reg. No./Substance:
0/Arabidopsis Proteins

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