| Gold nanoparticle-based colorimetric assay for the direct detection of cancerous cells. | |
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MedLine Citation:
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PMID: 18198894 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Early and accurate detection of cancer often requires time-consuming techniques and expensive instrumentation. To address these limitations, we developed a colorimetric assay for the direct detection of diseased cells. The assay uses aptamer-conjugated gold nanoparticles to combine the selectivity and affinity of aptamers and the spectroscopic advantages of gold nanoparticles to allow for the sensitive detection of cancer cells. Samples with the target cells present exhibited a distinct color change while nontarget samples did not elicit any change in color. The assay also showed excellent sensitivity with both the naked eye and based on absorbance measurements. In addition, the assay was able to differentiate between different types of target and control cells based on the aptamer used in the assay indicating the wide applicability of the assay for diseased cell detection. On the basis of these qualities, aptamer-conjugated gold nanoparticles could become a powerful tool for point of care diagnostics. |
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Authors:
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Colin D Medley; Joshua E Smith; Zhiwen Tang; Yanrong Wu; Suwussa Bamrungsap; Weihong Tan |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. Date: 2008-01-17 |
Journal Detail:
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Title: Analytical chemistry Volume: 80 ISSN: 0003-2700 ISO Abbreviation: Anal. Chem. Publication Date: 2008 Feb |
Date Detail:
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Created Date: 2008-02-14 Completed Date: 2008-05-07 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0370536 Medline TA: Anal Chem Country: United States |
Other Details:
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Languages: eng Pagination: 1067-72 Citation Subset: IM |
Affiliation:
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Center for Research at the Bio/Nano Interface, Department of Chemistry and Shands Cancer Center, UF Genetics Institute and McKnight Brain Institute, University of Florida, Gainesville, FL 32611, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Aptamers, Nucleotide
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chemistry* Base Sequence Biosensing Techniques / methods* Colorimetry / methods* Gold / chemistry* Metal Nanoparticles / chemistry* Microscopy, Electron, Transmission / methods Neoplasms / pathology*, ultrastructure Sensitivity and Specificity |
| Grant Support | |
ID/Acronym/Agency:
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2R01 GM 066137/GM/NIGMS NIH HHS; R21 CA 122648/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Aptamers, Nucleotide; 7440-57-5/Gold |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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