Document Detail


Gold Nanoparticles as a Probe for Prion Determination <i>via</i> Resonance Light Scattering Method.
MedLine Citation:
PMID:  22687927     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Dihydrolipoic acid (DHLA) modified gold nanoparticles (AuNPs) were used as a highly selective probe for the detection of prion proteins. We discovered that AuNPs undergo aggregation selectively in the presence of recombinant prion protein (rPrP), and such selective aggregation enhanced the resonance light scattering (RLS) intensity from AuNPs tremendously. Based on this phenomenon, we established a new assay for rPrP detection. This new assay is label-free, highly selective, and sensitive. The linear range for rPrP detection is from 2.0 × 10<sup>-10</sup> to 2.0 × 10<sup>-8</sup> mol L<sup>-1</sup> with excellent discrimination against other interfering compounds, and the detection limit is 7 × 10<sup>-11</sup> mol L<sup>-1</sup>. This assay has been successfully applied for rPrP detection in E. coli lysate, bovine serum samples and human plasma samples. Compared with other methods, the detection approach described here can achieve high selectivity and sensitivity without any complicated labeling or expensive instruments.
Authors:
Xiaoxiao Huang; Yijuan Long; Haijie Zhang; Qinlong Wang; Rui Zhu; Huzhi Zheng
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Analytical sciences : the international journal of the Japan Society for Analytical Chemistry     Volume:  28     ISSN:  1348-2246     ISO Abbreviation:  Anal Sci     Publication Date:  2012  
Date Detail:
Created Date:  2012-06-12     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8511078     Medline TA:  Anal Sci     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  475-9     Citation Subset:  IM    
Affiliation:
Key Laboratory on Luminescence and Real-Time Analysis (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University.
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