Document Detail


Gold nanoparticles capped with polyethyleneimine for enhanced siRNA delivery.
MedLine Citation:
PMID:  19924738     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
An efficient and safe delivery system for small interfering RNA (siRNA) is required for clinical application of RNA interfering therapeutics. Polyethyleneimine (PEI)-capped gold nanoparticles (AuNPs) are successfully manufactured using PEI as the reductant and stabilizer, which bind siRNA at an appropriate weight ratio by electrostatic interaction and result in well-dispersed nanoparticles with uniform structure and narrow size distribution. With siRNA binding, PEI-capped AuNPs induce more significant and enhanced reduction in targeted green fluorescent protein expression in MDA-MB-435s cells, though more internalized PEI/siRNA complexes in cells are evidenced by confocal laser scanning microscopy observation and fluorescence-activated cell sorting analyses. PEI-capped AuNPs/siRNA targeting endogenous cell-cycle kinase, an oncogene polo-like kinase 1 (PLK1), display significant gene expression knockdown and induce enhanced cell apoptosis, whereas it is not obvious when the cells are treated with PLK1 siRNA using PEI as the carrier. Without exhibiting cellular toxicity, PEI-capped AuNPs appear to be suitable as a potential carrier for intracellular siRNA delivery.
Authors:
Wen-Jing Song; Jin-Zhi Du; Tian-Meng Sun; Pei-Zhuo Zhang; Jun Wang
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Small (Weinheim an der Bergstrasse, Germany)     Volume:  6     ISSN:  1613-6829     ISO Abbreviation:  Small     Publication Date:  2010 Jan 
Date Detail:
Created Date:  2010-01-20     Completed Date:  2010-03-16     Revised Date:  2011-11-02    
Medline Journal Info:
Nlm Unique ID:  101235338     Medline TA:  Small     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  239-46     Citation Subset:  IM    
Affiliation:
Hefei National Laboratory for Physical Sciences at the Microscale, School of Life Sciences, University of Science and Technology of China Hefei, Anhui, P. R. China.
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MeSH Terms
Descriptor/Qualifier:
Apoptosis / drug effects
Blotting, Western
Cell Cycle Proteins / metabolism
Cell Line, Tumor
Gene Silencing / drug effects
Gene Transfer Techniques
Gold / chemistry*,  toxicity
Green Fluorescent Proteins / metabolism
Humans
Metal Nanoparticles / chemistry*,  toxicity
Polyethyleneimine / chemistry*,  toxicity
Protein-Serine-Threonine Kinases / metabolism
Proto-Oncogene Proteins / metabolism
RNA, Small Interfering / metabolism*
Chemical
Reg. No./Substance:
0/Cell Cycle Proteins; 0/Proto-Oncogene Proteins; 0/RNA, Small Interfering; 147336-22-9/Green Fluorescent Proteins; 7440-57-5/Gold; 9002-98-6/Polyethyleneimine; EC 2.7.11.1/Protein-Serine-Threonine Kinases; EC 2.7.11.1/polo-like kinase 1

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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