Document Detail

GnRH or eCG treatment fails to restore reproductive function in GnRH immunized ewes.
MedLine Citation:
PMID:  18534792     Owner:  NLM     Status:  MEDLINE    
This study was designed to evaluate the potential of using eCG or GnRH in restoring reproductive functions in GnRH immunized ewes. Thirty-three multiparous Kivircik ewes were randomly assigned into either control group (n=11) or immunization group (n=22). Ewes were immunized against GnRH by injecting with a cocktail of ovalbumin-LHRH-7 (ovalbumin-GnRH-7) and thioredoxin-LHRH-7 (thioredoxin-GnRH-7) fusion proteins generated by recombinant DNA technology in April. 500 IU eCG or 0.008 mg GnRH analogue was used to induce ovulations. Serum GnRH antibodies were present in animals of the immunized group beginning the second week after the first immunization and maintained throughout the study (14 months). Immunization caused anestrus in immunized ewes. eCG or GnRH analogue administration given after 14 days progestagen (20 mg fluorogestone acetate, FGA) treatment during breeding season (mid July) did not induce ovulation in these ewes. Two more attempts with single or multiple eCG injections failed to induce ovulation in this group as well. It appears that the gonadotropin stimulation was not of adequate time since neither eCG nor GnRH administration was able to restore reproductive function in immunized animals. The immunization effect lasted more than a year. These results suggest that GnRH immunization exerts its effect via the hypothalamo-pituitary axis and that more than such stimulation is required to overcome the reproductive suppression.
O Gökdal; O Atay; H Ulker; E Yarali; I B Helva; D M Deavila; J J Reeves
Publication Detail:
Type:  Controlled Clinical Trial; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.     Date:  2008-05-02
Journal Detail:
Title:  Animal reproduction science     Volume:  112     ISSN:  1873-2232     ISO Abbreviation:  Anim. Reprod. Sci.     Publication Date:  2009 Jun 
Date Detail:
Created Date:  2009-04-08     Completed Date:  2009-06-22     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7807205     Medline TA:  Anim Reprod Sci     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  251-60     Citation Subset:  IM    
Adnan Menderes University, Cine Vocational High School, 09500 Cine, Aydin, Turkey.
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MeSH Terms
Contraception / methods,  veterinary
Contraception, Immunologic / adverse effects,  veterinary
Estrous Cycle / drug effects
Estrus Synchronization / methods
Gonadotropin-Releasing Hormone / adverse effects,  immunology*,  therapeutic use*
Gonadotropins, Equine / therapeutic use*
Immunization / adverse effects,  veterinary
Infertility, Female / drug therapy*,  etiology*,  immunology
Recombinant Fusion Proteins / administration & dosage,  immunology
Recovery of Function / drug effects
Reproduction / drug effects*,  immunology
Sheep* / immunology,  physiology
Time Factors
Treatment Failure
Reg. No./Substance:
0/Gonadotropins, Equine; 0/Recombinant Fusion Proteins; 33515-09-2/Gonadotropin-Releasing Hormone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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