Document Detail

Glypican (heparan sulfate proteoglycan) is palmitoylated, deglycanated and reglycanated during recycling in skin fibroblasts.
MedLine Citation:
PMID:  9061369     Owner:  NLM     Status:  MEDLINE    
Skin fibroblasts treated with brefeldin A produce a recycling variant of glypican (a glycosylphosphatidylinositolanchored heparan-sulfate proteoglycan) that is resistant to inositol-specific phospholipase C and incorporates sulfate and glucosamine into heparan sulfate chains (Fransson, L.-A. et al., Glycobiology, 5, 407-415, 1995). We have now investigated structural modifications of recycling glypican, such as fatty acylation from [3H]palmitate, and degradation and assembly of heparan sulfate side chains. Most of the 3H-radioactivity was recovered as lipid-like material after de-esterification. To distinguish between formation of heparan sulfate at vacant sites, elongation of existing chains or degradation followed by re-elongation of chain remnants, cells were pulse-labeled with [3H]glucosamine and then chase-labeled with [14C]glucosamine. Material isolated from the cells during the chase consisted of proteoglycan and mostly [3H]-labeled heparan-sulfate degradation products (molecular mass, 20-80 kDa) showing that the side chains were degraded during recycling. The degradation products were initially glucuronate-rich, but became more iduronate-rich with time. The glypican proteoglycan formed during the chase was degraded either with alkali to release intact side chains or with heparinase to generate distally located chain fragments that were separated from the core protein, containing the proximally located, covalently attached chain remnants. All of the [14C]-radioactivity incorporated during the pulse was found in peripheral chain fragments, and the chains formed were not significantly longer than the original ones. We therefore conclude that newly made heparan-sulfate chains were neither made on vacant sites, nor by extension of existing chains but rather by re-elongation of degraded chain remnants. The remodeled chains made during recycling appeared to be more extensively modified than the original ones.
G Edgren; B Havsmark; M Jönsson; L A Fransson
Related Documents :
24534499 - Chemotaxis toward carbohydrates and peptides by mixed ruminal protozoa when fed, fasted...
12694919 - The presence of partially 3-o-methylated mannogalactan from the fruit bodies of edible ...
15047699 - A new model for the domain structure of heparan sulfate based on the novel specificity ...
4850989 - Chemical and physical properties of an arabinogalactan-peptide from wheat endosperm.
11585829 - Distinct carbohydrate recognition domains of an invertebrate defense molecule recognize...
2221389 - Quantitative analysis of n-sulfated, n-acetylated, and unsubstituted glucosamine amino ...
11045629 - Structure-based prediction of binding peptides to mhc class i molecules: application to...
24865469 - Molecular dynamics study of segment peptides of bax, bim, and mcl-1 bh3 domain of the a...
22508559 - Solution structure studies of monomeric human tip47/perilipin-3 reveal a highly extende...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Glycobiology     Volume:  7     ISSN:  0959-6658     ISO Abbreviation:  Glycobiology     Publication Date:  1997 Feb 
Date Detail:
Created Date:  1997-05-27     Completed Date:  1997-05-27     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9104124     Medline TA:  Glycobiology     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  103-12     Citation Subset:  IM    
Department of Cell and Molecular Biology, Faculty of Medicine, Lund University, Sweden.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Brefeldin A
Cells, Cultured
Cyclopentanes / pharmacology
Fibroblasts / drug effects,  metabolism
Heparan Sulfate Proteoglycans
Heparitin Sulfate / chemistry,  metabolism*
Monensin / pharmacology
Palmitic Acid / metabolism*
Polysaccharides / metabolism*
Proteoglycans / chemistry,  metabolism*
Skin / cytology,  drug effects,  metabolism*
Reg. No./Substance:
0/Cyclopentanes; 0/Heparan Sulfate Proteoglycans; 0/Polysaccharides; 0/Proteoglycans; 10028-17-8/Tritium; 17090-79-8/Monensin; 20350-15-6/Brefeldin A; 57-10-3/Palmitic Acid; 9050-30-0/Heparitin Sulfate

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  The fucosylation and secretion of mucins synthesized in human bronchial cells vary according to grow...
Next Document:  Isolation and characterization of a class II alpha-mannosidase cDNA from lepidopteran insect cells.