Document Detail

Glypican 3 has a higher sensitivity than alpha-fetoprotein for testicular and ovarian yolk sac tumour: immunohistochemical investigation with analysis of histological growth patterns.
MedLine Citation:
PMID:  20546340     Owner:  NLM     Status:  MEDLINE    
AIMS: Glypican 3 (GPC3) has been reported to be overexpressed in yolk sac tumour (YST), but the sensitivity has not been compared with alpha-fetoprotein (AFP). YST can form numerous growth patterns and the expression of GPC3 in these patterns has not been studied. The aim was to address these aspects.
METHODS AND RESULTS: Sections from testicular or ovarian YST were subjected to immunohistochemistry using GPC3 (n = 39) and AFP (n = 24). Overall immunoreactivity for each case and specific histological patterns were semiquantitatively evaluated (0-3+) and intensity of reactivity was scored (0-3). All cases expressed GPC3 (1+, 5%; 2+, 8%; 3+, 87%) with strong intensity (2.9). The majority expressed AFP (58%) but immunoreactivity was often focal (0, 42%; 1+, 33%; 2+, 25%) and intensity was low (1.0). Using GPC3, >75% of the microcystic (n = 38), macrocystic (n = 26), solid (n = 21), glandular-alveolar (n = 8), endodermal sinus (n = 7), polyvesicular vitelline (n = 5), enteric (n = 4) and micropapillary (n = 2) growth patterns displayed 2+ or 3+ positivity.
CONCLUSIONS: YST can display a variety of growth patterns that can be confused with other germ cell tumour components. GPC3 detects all growth patterns tested and has a higher sensitivity for detecting YST than AFP.
Debra L Zynger; John C McCallum; Chunyan Luan; Pauline M Chou; Ximing J Yang
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Histopathology     Volume:  56     ISSN:  1365-2559     ISO Abbreviation:  Histopathology     Publication Date:  2010 May 
Date Detail:
Created Date:  2010-06-15     Completed Date:  2010-11-03     Revised Date:  2011-03-21    
Medline Journal Info:
Nlm Unique ID:  7704136     Medline TA:  Histopathology     Country:  England    
Other Details:
Languages:  eng     Pagination:  750-7     Citation Subset:  IM    
Department of Pathology, The Ohio State University Medical Center, Columbus, OH, USA.
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MeSH Terms
Endodermal Sinus Tumor / metabolism,  pathology*
Glypicans / metabolism*
Neoplasms, Germ Cell and Embryonal / metabolism,  pathology*
Ovarian Neoplasms / metabolism,  pathology*
Sensitivity and Specificity
Testicular Neoplasms / metabolism,  pathology*
Testis / metabolism,  pathology
alpha-Fetoproteins / metabolism*
Reg. No./Substance:
0/Glypicans; 0/alpha-Fetoproteins
Comment In:
Histopathology. 2011 Jan;58(2):312-4; author reply 314-5   [PMID:  21255065 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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