Document Detail


Glyphosate-induced oxidative stress in rice leaves revealed by proteomic approach.
MedLine Citation:
PMID:  18755596     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Glyphosate is one of the most widely used herbicides in cereal-growing regions worldwide. In the present work, the protein expression profile of rice leaves exposed to glyphosate was analyzed in order to investigate the alternative effects of glyphosate on plants. Two-week-old rice leaves were subjected to glyphosate or a reactive oxygen species (ROS) inducing herbicide paraquat, and total soluble proteins were extracted and analyzed by two-dimensional gel electrophoresis (2-DE) coupled with matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) mass spectrometry (MS) analysis. A total of 25 differentially expressed proteins were identified from the glyphosate treated sample, wherein 18 proteins were up-regulated and 7 proteins were down-regulated. These proteins had shown a parallel expression pattern in response to paraquat. Results from the 2-DE analysis, combined with immunoblotting, clearly revealed that ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) large subunit was significantly decreased by the treatment of both herbicides. An increased accumulation of antioxidant enzymes including ascorbate peroxidase, glutathione S-transferase, thioredoxin h-type, nucleoside diphosphate kinase 1, peroxiredoxin and a superoxide dismutase [Cu-Zn] chloroplast precursor in the glyphosate-treated sample suggests that a glyphosate treatment possibly generates oxidative stress in plants. Moreover, a gene expression analysis of five antioxidant enzymes by Northern blot confirmed their mRNA levels in the rice leaves. A histo-cytochemical investigation with DAB (3,3-diaminobenzidine) to localize H(2)O(2) and increases of the thiobarbituric acid reactive substances (TBARS) concentration revealed that the glyphosate application generates ROS, which resulted in the peroxidation and destruction of lipids in the rice leaves.
Authors:
Nagib Ahsan; Dong-Gi Lee; Ki-Won Lee; Iftekhar Alam; Sang-Hoon Lee; Jeong Dong Bahk; Byung-Hyun Lee
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-07-19
Journal Detail:
Title:  Plant physiology and biochemistry : PPB / Société française de physiologie végétale     Volume:  46     ISSN:  0981-9428     ISO Abbreviation:  Plant Physiol. Biochem.     Publication Date:  2008 Dec 
Date Detail:
Created Date:  2008-11-10     Completed Date:  2009-02-19     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9882449     Medline TA:  Plant Physiol Biochem     Country:  France    
Other Details:
Languages:  eng     Pagination:  1062-70     Citation Subset:  IM    
Affiliation:
Division of Applied Life Sciences (BK21 Program), Gyeongsang National University, Jinju 660-701, Republic of Korea.
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MeSH Terms
Descriptor/Qualifier:
Base Sequence
Blotting, Western
DNA Primers
Glycine / analogs & derivatives*,  pharmacology
Oryza sativa / drug effects*,  enzymology,  metabolism
Oxidative Stress / drug effects*
Photosynthesis
Plant Leaves / drug effects*,  enzymology,  metabolism
Proteomics*
RNA, Messenger / genetics
Reactive Oxygen Species / metabolism
Signal Transduction
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Chemical
Reg. No./Substance:
0/DNA Primers; 0/RNA, Messenger; 0/Reactive Oxygen Species; 1071-83-6/glyphosate; 56-40-6/Glycine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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