Document Detail

Glycyl-L-sarcosine absorption across ovine omasal epithelium during coincubation with other peptide substrates and volatile fatty acids.
MedLine Citation:
PMID:  9814913     Owner:  NLM     Status:  MEDLINE    
To define the interactions between the absorption of glycyl-L-sarcosine (Gly-Sar; .1 mM) and glycine, L-methionylglycine, glycyl-L-leucine, L-carnosine, or L-methionylglycyl-L-methionyl-L-methionine (each at 5 mM), ovine omasal epithelium was collected from eight wethers (average BW=69+/-8.2 kg) and mounted in parabiotic chambers. [1,2]-[14C]Glycyl-L-sarcosine was used as a marker to monitor the presence of Gly-Sar. The Gly-Sar concentration in the omasal epithelium after 60 min of incubation was greatest (P < .05; .0055 nmol/mg dry tissue) when only Gly-Sar was present. Glycine inhibited (P < .05) Gly-Sar movement through the tissue by 20%, and peptide substrates inhibited (P < .05) Gly-Sar movement by 60 to 85%. The appearance of Gly-Sar in serosal buffers increased quadratically (P < .001) with time. Numerically, Gly-Sar appearance in serosal buffers was stimulated by the presence of glycine and peptide substrates. In a second experiment, ovine omasal epithelium was collected from four lambs (average BW=47+/-6.0 kg) to determine the interactions of Gly-Sar absorption (.1 mM) alone or when coincubated with either 10 mM butyric acid, or with a mixture of VFA (50 mM acetic acid, 40 mM propionic acid, and 10 mM butyric acid). The movement of Gly-Sar through the omasal epithelium was greatest (P < .05) when only Gly-Sar was present, and the VFA mixture inhibited (P < .05) Gly-Sar movement by 84%. Results from these studies support the idea that peptides can be absorbed across omasal epithelium and that the process involves mediated as well as nonmediated mechanisms, including possibly paracellular transport.
M Q McCollum; K E Webb
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of animal science     Volume:  76     ISSN:  0021-8812     ISO Abbreviation:  J. Anim. Sci.     Publication Date:  1998 Oct 
Date Detail:
Created Date:  1999-02-01     Completed Date:  1999-02-01     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8003002     Medline TA:  J Anim Sci     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  2706-11     Citation Subset:  IM    
Department of Animal and Poultry Sciences, Virginia Polytechnic Institute and State University, Blacksburg 24061-0306, USA.
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MeSH Terms
Carnosine / metabolism
Dipeptides / metabolism,  pharmacokinetics*
Epithelium / metabolism
Fatty Acids, Volatile / metabolism*
Glycine / metabolism
Omasum / metabolism*
Peptides / metabolism*
Sheep / metabolism*
Reg. No./Substance:
0/Dipeptides; 0/Fatty Acids, Volatile; 0/Peptides; 14486-03-4/methionylglycine; 29816-01-1/glycylsarcosine; 305-84-0/Carnosine; 56-40-6/Glycine; 869-19-2/glycylleucine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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