Document Detail


Glycosylation potential of human prostate cancer cell lines.
MedLine Citation:
PMID:  22843320     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Altered glycosylation is a universal feature of cancer cells and altered glycans can help cancer cells escape immune surveillance, facilitate tumor invasion, and increase malignancy. The goal of this study was to identify specific glycoenzymes, which could distinguish prostate cancer cells from normal prostatic cells. We investigated enzymatic activities and gene expression levels of key glycosyl- and sulfotransferases responsible for the assembly of O- and N-glycans in several prostatic cells. These cells included immortalized RWPE-1 cells derived from normal prostatic tissues, and prostate cancer cells derived from metastasis in bone (PC-3), brain (DU145), lymph node (LNCaP), and vertebra (VCaP). We found that all cells were capable of synthesizing complex N-glycans and O-glycans with the core 1 structure, and each cell line had characteristic biosynthetic pathways to modify these structures. The in vitro measured activities corresponded well to the mRNA levels of glycosyltransferases and sulfotransferases. Lectin and antibody binding to whole cells supported these results, which form the basis for the development of tumor cell-specific targeting strategies.
Authors:
Yin Gao; Vishwanath B Chachadi; Pi-Wan Cheng; Inka Brockhausen
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2012-07-28
Journal Detail:
Title:  Glycoconjugate journal     Volume:  29     ISSN:  1573-4986     ISO Abbreviation:  Glycoconj. J.     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-09-05     Completed Date:  2013-01-15     Revised Date:  2014-08-17    
Medline Journal Info:
Nlm Unique ID:  8603310     Medline TA:  Glycoconj J     Country:  United States    
Other Details:
Languages:  eng     Pagination:  525-37     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Caco-2 Cells
Glycoproteins / genetics,  metabolism*
Glycosylation
Glycosyltransferases / genetics,  metabolism*
Humans
Male
Neoplasm Metastasis
Neoplasm Proteins / genetics,  metabolism*
Prostatic Neoplasms / genetics,  metabolism*,  pathology
Sulfotransferases / genetics,  metabolism*
Grant Support
ID/Acronym/Agency:
1R21HL097238/HL/NHLBI NIH HHS; 2R01HL48282/HL/NHLBI NIH HHS; R01 HL048282/HL/NHLBI NIH HHS; R21 HL097238/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Glycoproteins; 0/Neoplasm Proteins; EC 2.4.-/Glycosyltransferases; EC 2.8.2.-/Sulfotransferases
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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