Document Detail

Glycosylation of interleukin-6 purified from normal human blood mononuclear cells.
MedLine Citation:
PMID:  1730219     Owner:  NLM     Status:  MEDLINE    
Interleukin 6 (IL-6) is a glycosylated cytokine which is important in exerting cell-specific growth-inducing, growth-inhibiting and differentiation-inducing effects. IL-6 produced in mammalian cell lines is heterogeneous, reflecting specific cell-type-dependent post-translational modifications. Native IL-6 was purified from human blood mononuclear cells and the oligosaccharides released, radiolabelled and sequenced by a combination of sequential exoglycosidase digestion using Bio-Gel P-4 high-resolution gel chromatography and acetolysis. N- and O-linked glycans were found. The N-linked glycans were sialylated di- and tri-antennary complex-type and oligomannose-type structures. However, the most predominant N-linked oligosaccharide was a small tetrasaccharide with the sequence Man alpha 6Man beta 4GlcNAc beta 4GlcNAc. This is the first report of this structure on a circulating glycoprotein. This structure has only previously been reported to be present on the syncytiotrophoblast of human placenta. The presence of the oligomannose structures and the mannose-terminating tetrasaccharide on IL-6 may be important in maintaining a high local concentration of the cytokine while limiting its systemic serum level via interaction with soluble mannose-binding serum lectins.
R B Parekh; R A Dwek; T W Rademacher; G Opdenakker; J Van Damme
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  European journal of biochemistry / FEBS     Volume:  203     ISSN:  0014-2956     ISO Abbreviation:  Eur. J. Biochem.     Publication Date:  1992 Jan 
Date Detail:
Created Date:  1992-02-19     Completed Date:  1992-02-19     Revised Date:  2007-07-23    
Medline Journal Info:
Nlm Unique ID:  0107600     Medline TA:  Eur J Biochem     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  135-41     Citation Subset:  IM    
Department of Biochemistry, Oxford, England.
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MeSH Terms
Carbohydrate Sequence
Chromatography, Gel
Electrophoresis, Polyacrylamide Gel
Interleukin-6 / isolation & purification,  metabolism*
Leukocytes, Mononuclear / metabolism*
Molecular Sequence Data
Protein Processing, Post-Translational
Reg. No./Substance:

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