Document Detail

Glycosylation of amphipathic lactoside primers with consequent inhibition of endogenous glycosphingolipid synthesis.
MedLine Citation:
PMID:  9644257     Owner:  NLM     Status:  MEDLINE    
The incubation of amphipathic lactosides with cultured cells was found to prime the glycosylation of lactosides whose oligosaccharide structures were exactly the same as those of glycosphingolipids produced by cells: B16 melanoma cells produced alpha2-3 sialylated lactosides; and PC12 cells, Galalpha1-4- and Galalpha1-3Galalpha1-4lactosides. Analysis of the cell-associated glycosylated products indicated that C16 series lactoside primers function 5-6 times more efficiently as acceptors than C12 series primers. The glycosylated lactosides were also secreted into the culture medium. Lactoside primers with longer hydrophobic chains hampered the release of glycosylated products from cells. The presence of an N-acyl chain in the lipophilic moiety of primers suppressed the secretion of glycosylated products. Owing to its overall availability, lactosides with the C12 alkyl chain were glycosylated 2-3 times more than C16 series lactosides and 1.4 times more than lactosides with the C12 acyl chain. C8-lactosides did not function as primers under the conditions of this study, but they were found to be the best acceptors for sialic acid transfer with the soluble enzyme fraction. The incubation of cells with 10 microM N-hexadecanoylaminoethyl-beta-O-lactoside caused a 30% decrease in endogenous GM3 of B16 cells and a 34% decrease in Gb3Cer synthesis of PC12 cells. The results of the present study demonstrate that lactoside primers serve as an efficient means to inhibit endogenous glycosphingolipids in studies to clarify glycosphingolipid functions.
H Nakajima; Y Miura; T Yamagata
Related Documents :
6272167 - Stimulation of resistance to 6-hydroxydopamine in a human neuroblastoma cell line by ne...
8959337 - Nm23 influences proliferation and differentiation of pc12 cells in response to nerve gr...
17409477 - Neurite outgrowth and branching of pc12 cells on very soft substrates sharply decreases...
14514037 - Effect of agmatine against cell death induced by nmda and glutamate in neurons and pc12...
12775567 - Role of macrophages in the generation of circulating blood nucleosomes from dead and dy...
11640947 - Altered subcellular distribution of transcriptional regulators in response to abeta pep...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of biochemistry     Volume:  124     ISSN:  0021-924X     ISO Abbreviation:  J. Biochem.     Publication Date:  1998 Jul 
Date Detail:
Created Date:  1998-09-21     Completed Date:  1998-09-21     Revised Date:  2007-12-19    
Medline Journal Info:
Nlm Unique ID:  0376600     Medline TA:  J Biochem     Country:  JAPAN    
Other Details:
Languages:  eng     Pagination:  148-56     Citation Subset:  IM    
Department of Biomolecular Engineering, Tokyo Institute of Technology, Midori-ku, Yokohama, 226-8501, Japan.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Cell Division / drug effects
Culture Media
Glycosides / metabolism*,  pharmacology
Glycosphingolipids / antagonists & inhibitors,  biosynthesis*
Melanoma, Experimental / metabolism,  pathology
PC12 Cells
Sialyltransferases / metabolism
Tumor Cells, Cultured
Reg. No./Substance:
0/Culture Media; 0/Glycosides; 0/Glycosphingolipids; 0/lactosides; EC 2.4.99.-/Sialyltransferases; EC alpha-2,3-sialyltransferase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Substrate specificities and kinetic properties of proteinase A from the yeast Saccharomyces cerevisi...
Next Document:  Kinetic characterization of lysine-specific metalloendopeptidases from Grifola frondosa and Pleurotu...