Document Detail

Glycosylated hemoglobin measured by affinity chromatography: micro-sample collection and room-temperature storage.
MedLine Citation:
PMID:  6851097     Owner:  NLM     Status:  MEDLINE    
Under proper conditions, whole blood can be stored at room temperature for as long as 21 days before measurement of glycosylated hemoglobin by affinity chromatography. Whole blood (anticoagulated with EDTA or heparin) was placed in capillary tubes, which were then sealed at both ends and stored at room temperature. Just before assay, whole blood was rinsed from the tubes and diluted 10-fold with water. Samples of each patient's blood were assayed as whole-blood hemolysates by affinity chromatography after zero, seven, 14, and 21 days of storage. Values for glycosylated hemoglobin did not change over 21 days of storage and values for each storage day correlated well (r = 0.97, p less than .0001) with hemoglobin A1C measured in fresh erythrocyte hemolysates by "high-performance" liquid ion-exchange chromatography.
R R Little; J D England; H M Wiedmeyer; D E Goldstein
Related Documents :
7092797 - Identification of eleven human hemoglobin variants by high-performance liquid chromatog...
7363467 - Accuracy, precision, and stability in measurement of hemoglobin a1c by "high-performanc...
9105267 - Capillary electrophoresis system for hemoglobin a1c determinations evaluated.
10374077 - Evaluation of ready-to-use liquid reagents for clinical chemistry in laboratory animals.
4086427 - Detection of tricresyl phosphates and determination of tri-o-cresyl phosphate in edible...
1702667 - Microdetermination of selenium in protein fractions isolated by analytical methods.
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Clinical chemistry     Volume:  29     ISSN:  0009-9147     ISO Abbreviation:  Clin. Chem.     Publication Date:  1983 Jun 
Date Detail:
Created Date:  1983-07-15     Completed Date:  1983-07-15     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  9421549     Medline TA:  Clin Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1080-2     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Blood Specimen Collection
Chromatography, Affinity
Chromatography, High Pressure Liquid
Chromatography, Ion Exchange
Diabetes Mellitus / blood*
Hemoglobin A, Glycosylated / analysis*
Specimen Handling
Time Factors
Grant Support
Reg. No./Substance:
0/Hemoglobin A, Glycosylated

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Reagent for the enzymatic determination of serum total cholesterol with improved lipolytic efficienc...
Next Document:  Liquid-chromatographic study of fluorescent materials in uremic fluids.