Document Detail


Glycosylated hemoglobin measured by affinity chromatography: micro-sample collection and room-temperature storage.
MedLine Citation:
PMID:  6851097     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Under proper conditions, whole blood can be stored at room temperature for as long as 21 days before measurement of glycosylated hemoglobin by affinity chromatography. Whole blood (anticoagulated with EDTA or heparin) was placed in capillary tubes, which were then sealed at both ends and stored at room temperature. Just before assay, whole blood was rinsed from the tubes and diluted 10-fold with water. Samples of each patient's blood were assayed as whole-blood hemolysates by affinity chromatography after zero, seven, 14, and 21 days of storage. Values for glycosylated hemoglobin did not change over 21 days of storage and values for each storage day correlated well (r = 0.97, p less than .0001) with hemoglobin A1C measured in fresh erythrocyte hemolysates by "high-performance" liquid ion-exchange chromatography.
Authors:
R R Little; J D England; H M Wiedmeyer; D E Goldstein
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Clinical chemistry     Volume:  29     ISSN:  0009-9147     ISO Abbreviation:  Clin. Chem.     Publication Date:  1983 Jun 
Date Detail:
Created Date:  1983-07-15     Completed Date:  1983-07-15     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  9421549     Medline TA:  Clin Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1080-2     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Blood Specimen Collection
Chromatography, Affinity
Chromatography, High Pressure Liquid
Chromatography, Ion Exchange
Diabetes Mellitus / blood*
Hemoglobin A, Glycosylated / analysis*
Humans
Methods
Specimen Handling
Temperature
Time Factors
Grant Support
ID/Acronym/Agency:
HL-13632/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Hemoglobin A, Glycosylated

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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