Document Detail


Glycosylated carriers for cell-selective and nuclear delivery of nucleic acids.
MedLine Citation:
PMID:  21622215     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Targeted gene delivery via selective cellular receptors has been realized as a crucial strategy for successful gene therapy by maximizing therapeutic efficiency in target cells and minimizing systemic toxicity. The membrane carbohydrate-binding proteins (membrane lectins) with different carbohydrate specificities are differentially expressed on the cellular and intracellular membranes of a number of cells. Their multiplicity, high affinity, and effective endocytosis after receptor binding as well as the biocompatibility of carbohydrate ligands endow them as potential ligands for glycosylated carriers in cell-selective delivery of nucleic acids. To achieve the in vivo application, glycosylated carriers/nucleic acid complexes have to fulfill certain conditions, including having a suitable size, minimal nonspecific interactions, low immunogenicity, and high uptake in target cells. Accordingly, the effective nuclear delivery of nucleic acids is the paramount important step for efficient gene transfer. This review summarizes the recent progress regarding application of glycosylated carriers for cell-selective and nuclear delivery of nucleic acids and their critical factors for efficient gene transfer. In addition, the development of new materials, such as carbon nanotubes, carbon nanospheres, and gold nanoparticles, as innovative carriers will be discussed with regards to glycosylation-mediated delivery of nucleic acids.
Authors:
Wassana Wijagkanalan; Shigeru Kawakami; Mitsuru Hashida
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Review     Date:  2011-06-01
Journal Detail:
Title:  Frontiers in bioscience : a journal and virtual library     Volume:  17     ISSN:  1093-4715     ISO Abbreviation:  Front. Biosci.     Publication Date:  2012  
Date Detail:
Created Date:  2011-05-30     Completed Date:  2011-10-03     Revised Date:  2012-01-10    
Medline Journal Info:
Nlm Unique ID:  9709506     Medline TA:  Front Biosci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2970-87     Citation Subset:  IM    
Affiliation:
Department of Drug Delivery Research, Graduate School of Pharmaceutical Sciences, Kyoto University, Kyoto 606-8501, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Nucleus / genetics
Dendritic Cells / drug effects,  metabolism
Drug Carriers* / chemistry
Endosomes / metabolism
Gene Therapy / methods*
Gene Transfer Techniques
Glycosylation
Hepatic Stellate Cells / drug effects,  metabolism
Hepatocytes / drug effects,  metabolism
Humans
Lung / cytology,  drug effects,  metabolism
Macrophages / drug effects,  metabolism
Models, Biological
Neoplasms / genetics,  therapy
Nucleic Acids / administration & dosage*,  genetics*
Targeted Gene Repair / methods
Chemical
Reg. No./Substance:
0/Drug Carriers; 0/Nucleic Acids

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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