Document Detail

Glycosyl-sn-1,2-dimyristylphosphatidylinositol is the membrane anchor for Trypanosoma equiperdum and T. (Nannomonas) congolense variant surface glycoproteins.
MedLine Citation:
PMID:  2957588     Owner:  NLM     Status:  MEDLINE    
We have analysed the structures of the Trypanosoma (Nannomonas) congolense and T. equiperdum variant surface glycoprotein (VSG) membrane anchors. Myristic acid uptake, phospholipase treatment, and nitrous acid deamination showed that, for each species, the anchor is glycosyl-sn-1,2-dimyristylphosphatidylinositol, as has been previously described for T. brucei. Osmotic lysis of these trypanosomes resulted in the release of soluble VSG, lacking fatty acid. In both species and in T. evansi, an endogenous phospholipase C, which cleaved diacylglycerol from membrane form VSG, was identified.
G S Lamont; J A Fox; G A Cross
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Molecular and biochemical parasitology     Volume:  24     ISSN:  0166-6851     ISO Abbreviation:  Mol. Biochem. Parasitol.     Publication Date:  1987 Jun 
Date Detail:
Created Date:  1987-10-20     Completed Date:  1987-10-20     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8006324     Medline TA:  Mol Biochem Parasitol     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  131-6     Citation Subset:  IM    
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MeSH Terms
Electrophoresis, Polyacrylamide Gel
Glycolipids / analysis*
Glycoproteins / analysis*
Myristic Acid
Myristic Acids / metabolism
Nitrous Acid / metabolism
Phosphatidylinositols / analysis*
Phospholipases / metabolism
Trypanosoma / analysis*
Trypanosoma brucei brucei / analysis
Trypanosoma congolense / analysis*
Variant Surface Glycoproteins, Trypanosoma
Grant Support
Reg. No./Substance:
0/Glycolipids; 0/Glycoproteins; 0/Glycosylphosphatidylinositols; 0/Myristic Acids; 0/Phosphatidylinositols; 0/Variant Surface Glycoproteins, Trypanosoma; 544-63-8/Myristic Acid; 7782-77-6/Nitrous Acid; EC 3.1.-/Phospholipases

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