Document Detail


Glycosphingolipids of K562 cells: a chemical and immunological analysis.
MedLine Citation:
PMID:  7033147     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The K562 cell line, which was established from a patient with chronic myeloid leukemia in blast crisis, was thought to be myeloid, but recent data indicate that it is an undifferentiated erythroid cell line. We have found that the glycosphingolipid content of these cells differs considerably from that of mature erythrocytes. Globotetraosylceramide, the most abundant glycolipid of mature red cells, was not detected in K562 cells, and neither was globotriaosylceramide. The predominant neutral glycolipids of K562 cells are monohexaosylceramides, which are a mixture of glucosyl- and galactosylceramides, and lactotriaosyl- and lactoneotetraosylceramides were also detected. Secondly, gangliosides which contain N-acetylgalactosamine were much more abundant than those containing N-acetylglucosamine in K562 cells, in contrast to erythrocytes. The most abundant ganglioside of K562 cells, GM2, is present in trace quantities in erythrocytes. A third major difference between these two cells lies in their relative proportions of neutral glycolipids and gangliosides. The molar ratio of neutral glycolipids/gangliosides is approximately 15:1 in erythrocytes and 1:1 in K562 cells. These striking differences between K562 cells and mature erythrocytes indicate that glycolipids may be useful cell surface markers of normal erythrocyte differentiation, and of erythroleukemias.
Authors:
A Suzuki; R A Karol; S K Kundu; D M Marcus
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  International journal of cancer. Journal international du cancer     Volume:  28     ISSN:  0020-7136     ISO Abbreviation:  Int. J. Cancer     Publication Date:  1981 Sep 
Date Detail:
Created Date:  1982-03-13     Completed Date:  1982-03-13     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0042124     Medline TA:  Int J Cancer     Country:  DENMARK    
Other Details:
Languages:  eng     Pagination:  271-6     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Adult
Cell Line
Chromatography, Thin Layer
Erythrocytes / analysis*
Female
Fetal Blood / analysis
Fluorescent Antibody Technique
Gangliosides / analysis
Glycolipids / analysis
Glycosphingolipids / analysis*
Humans
Leukemia, Myeloid, Acute / analysis*
Pregnancy
Trypsin / metabolism
Grant Support
ID/Acronym/Agency:
1F32 CA 06469/CA/NCI NIH HHS; AI 17712/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/Gangliosides; 0/Glycolipids; 0/Glycosphingolipids; EC 3.4.21.4/Trypsin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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