Document Detail

Glycosphingolipid patterns in human promyelocytic HL-60 leukemia cells susceptible or resistant to differentiation induction by phorbol 12-myristate 13-acetate.
MedLine Citation:
PMID:  8452876     Owner:  NLM     Status:  MEDLINE    
The patterns of glycosphingolipids (GSLs) were analyzed in an HL-60 cell variant, HL-205, which is susceptible to phorbol 12-myristate 13-acetate (PMA)-induced monocyte/macrophage differentiation, and in an HL-60 cell variant, HL-525, which is resistant to such differentiation. The amounts and types of the GSLs were similar in both the HL-205 and HL-525 cells and they resemble those of granulocytes. Treatment with 3 nM PMA caused the susceptible HL-205 cells (but not the resistant cells) to acquire a new GSL pattern which resembles that of monocytes. This new pattern was characterized by increases in the level of a neutral GSL, Gb3Cer, from trace levels to 0.05 mg/10(9) cells and of an acidic GSL, GM3 ganglioside, from 0.03 to 0.33 mg/10(9) cells. The increases in the level of this ganglioside were found to be due to an increase in CMP-N-acetylneuraminic acid:lactosylceramide sialyltransferase activity. These results indicate an association between PMA-induced terminal differentiation along the monocyte/macrophage cell lineage and PMA-evoked increases in specific GSLs, GM3 in particular, which is due to a rise in the activity of its synthetic enzyme.
K Kiguchi; C Henning-Chubb; E Huberman
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  1176     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  1993 Mar 
Date Detail:
Created Date:  1993-04-16     Completed Date:  1993-04-16     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  27-36     Citation Subset:  IM    
Biological and Medical Research Division, Argonne National Laboratory, Illinois 60439.
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MeSH Terms
Carbohydrate Sequence
Cell Differentiation / drug effects
Enzyme Induction / drug effects
G(M3) Ganglioside / biosynthesis
Glycosphingolipids / biosynthesis*,  chemistry
Leukemia, Promyelocytic, Acute / genetics
Molecular Sequence Data
Monocytes / metabolism
Sialyltransferases / metabolism
Tetradecanoylphorbol Acetate / pharmacology*
Tumor Cells, Cultured / drug effects*
Reg. No./Substance:
0/G(M3) Ganglioside; 0/Glycosphingolipids; 16561-29-8/Tetradecanoylphorbol Acetate; EC 2.4.99.-/Sialyltransferases; EC alpha 2-6-sialyltransferase

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