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Glycosidic intermediates identified in (1) H MR spectra of intact tumour cells may contribute to the clarification of aspects of glycosylation pathways.
MedLine Citation:
PMID:  20669171     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
The glycosylation process, through the addition of carbohydrates, is a major post-translational modification of proteins and glycolipids. Proteins may be glycosylated in either the secretory pathway leading to N-linked or O-linked glycoproteins or as nucleocytoplasmic glycosylation that targets only single proteins involving a single β-linked N-acetylglucosamine. In both cases, the key precursors are the uridine diphospho-N-acetylhexosamines synthesised by the hexosamine biosynthetic pathway. Furthermore, uridine diphospho-N-acetylglucosamine participates in the biosynthesis of sialic acid. In this work, we propose MRS for the detection of uridine diphospho-N-acetylhexosamines visible in high-resolution MR spectra of intact cells from different human tumours. Signals from the nucleotide and amino sugar moieties, including amide signals observed for the first time in whole cells, are assigned, also taking advantage of spectral changes that follow cell treatment with ammonium chloride. Finally, parallel changes in uridine diphospho-N-acetylhexosamines and glutamine pools, observed after pH changes induced by ammonium chloride in the different tumour cell lines, may provide more details on the glycosylation processes. Copyright © 2010 John Wiley & Sons, Ltd.
Authors:
Sveva Grande; Alessandra Palma; Anna Maria Luciani; Antonella Rosi; Laura Guidoni; Vincenza Viti
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  NMR in biomedicine     Volume:  24     ISSN:  1099-1492     ISO Abbreviation:  NMR Biomed     Publication Date:  2011 Jan 
Date Detail:
Created Date:  2011-01-24     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8915233     Medline TA:  NMR Biomed     Country:  England    
Other Details:
Languages:  eng     Pagination:  68-79     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 John Wiley & Sons, Ltd.
Affiliation:
Dipartimento di Tecnologie e Salute and INFN Gruppo Collegato Sanità, Istituto Superiore di Sanità, Rome, Italy. sveva.grande@iss.it.
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