Document Detail


Glycosaminoglycans in the pericellular matrix of chondrons and chondrocytes.
MedLine Citation:
PMID:  18631286     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
This is the first study to quantitate and profile the glycosaminoglycan (GAG) composition of the pericellular matrix (PCM) of chondrons and chondrocytes using the highly sensitive technique; fluorophore-assisted carbohydrate electrophoresis (FACE). Bovine articular chondrocytes and chondrons were isolated enzymatically. High cell yield and viability were obtained for both preparations. Chondrons had strong immunofluorescent labeling for keratan sulphate and chondroitin-6 sulphate but no labeling for hyaluronan. We compared the immunofluorescent data with FACE. The quantities of total keratan sulphate were determined to be 0.013 +/- 0.002 pg cell(-1) and 0.032 +/- 0.003 pg cell(-1) in the chondrocyte and chondron preparations, respectively. Four internal keratan sulphate sugars were detected (gal beta 1,4glcNAc6S, gal6S beta 1,4glcNAc6S, glcNAc beta 1,3gal and glcNAc6S beta 1,3gal) for both preparations but they were present at significantly higher concentrations in chondron preparations (P < 0.01). Total chondroitin sulphate (CS) was determined to be 0.054 +/- 0.004 pg cell(-1) and 0.077 +/- 0.005 pg cell(-1) for chondrocyte and chondron preparations, respectively. Unsulphated CS disaccharide levels were similar but chondrons had significantly more chondroitin-4 sulphated disaccharides and chondroitin-6 sulphated disaccharides (P < 0.05). Hyaluronan acid was present at low concentrations (0.010 +/- 0.001 pg cell(-1)) in both chondrocytes and chondrons. In this study, enzyme digestion coupled with FACE separation revealed new information about the differences in GAGs from isolated chondrocyte and chondron preparations. Further investigation of the differences in GAGs from chondrocytes and chondrons from different zones of articular cartilage may be useful for tissue engineering approaches.
Authors:
Qi Guang Wang; Alicia J El Haj; Nicola J Kuiper
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-07-08
Journal Detail:
Title:  Journal of anatomy     Volume:  213     ISSN:  1469-7580     ISO Abbreviation:  J. Anat.     Publication Date:  2008 Sep 
Date Detail:
Created Date:  2008-10-10     Completed Date:  2008-12-04     Revised Date:  2013-06-05    
Medline Journal Info:
Nlm Unique ID:  0137162     Medline TA:  J Anat     Country:  England    
Other Details:
Languages:  eng     Pagination:  266-73     Citation Subset:  IM    
Affiliation:
Institute for Science & Technology in Medicine, University of Keele, Huxley Building, Staffordshire, ST5 5BG, UK.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cartilage, Articular / chemistry*,  cytology
Cattle
Cell Count
Cell Separation / methods
Chondrocytes / chemistry*
Electrophoresis / methods
Extracellular Matrix / chemistry*
Extracellular Matrix Proteins / analysis
Female
Flow Cytometry
Fluorescent Dyes
Glycosaminoglycans / analysis*
Immunohistochemistry
Keratan Sulfate / analysis
Tissue Engineering
Chemical
Reg. No./Substance:
0/Extracellular Matrix Proteins; 0/Fluorescent Dyes; 0/Glycosaminoglycans; 9056-36-4/Keratan Sulfate
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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