| Glycosaminoglycan synthesis is depressed during mitosis and elevated during early G1. | |
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MedLine Citation:
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PMID: 4040916 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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[35S]Sulfate incorporation was measured in populations of Chinese hamster ovary cells enriched for mitotics, early G1 cells, and interphase monolayers or suspensions. Incorporation was determined by biochemical analysis of extracts and quantitative autoradiography of thick sections. 90% of [35S]sulfate was incorporated into glycosaminoglycan (GAG). Incorporation was depressed fourfold in mitotics and stimulated by from two- to three-fold in early G1 cells relative to mixed interphase cells. GAG synthesis was maintained into late G2. Thus, the rate of GAG biosynthesis was correlated temporally with the detachment and reattachment of cells to substrate. Inhibitors of protein synthesis brought about the rapid arrest of GAG biosynthesis. However, xylosides, which bypass the requirement for core protein, did not bring oligosaccharide sulfation in mitotics to interphase levels. These observations indicate an inhibition of Golgi processing and are consistent with a generalized defect of membrane vesicle-mediated transport during mitosis. |
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Authors:
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S F Preston; C S Regula; P R Sager; C B Pearson; L S Daniels; P A Brown; R D Berlin |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: The Journal of cell biology Volume: 101 ISSN: 0021-9525 ISO Abbreviation: J. Cell Biol. Publication Date: 1985 Sep |
Date Detail:
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Created Date: 1985-10-09 Completed Date: 1985-10-09 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 0375356 Medline TA: J Cell Biol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1086-93 Citation Subset: IM |
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Adhesion Cell Line Cricetinae Cricetulus Female Glycosaminoglycans / biosynthesis* Glycosides / pharmacology Guinea Pigs Interphase* Mitosis* Ovary Protein Biosynthesis Sulfates / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Glycosaminoglycans; 0/Glycosides; 0/Sulfates; 0/xylosides |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
| Full Text | |
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Journal Information Journal ID (nlm-ta): J Cell Biol ISSN: 0021-9525 ISSN: 1540-8140 Publisher: The Rockefeller University Press |
Article Information Download PDF  Print publication date: Day: 1 Month: 9 Year: 1985 Volume: 101 Issue: 3 First Page: 1086 Last Page: 1093 ID: 2113704 Publisher Id: 85289486 PubMed Id: 4040916 |
| Glycosaminoglycan synthesis is depressed during mitosis and elevated during early G1 | |
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