| Glycosaminoglycan fractions in normal human urine. | |
| | |
MedLine Citation:
|
PMID: 15637905 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
A study has been made of glycosaminoglycans in normal urine to determine which are non-dialysable, which are ultrafilterable, and which are precipitable with cetypyridinium chloride or alcohol. The main fractions present in human urine are: (1) High molecular weight material which is non-dialysable and precipitated by alcohol and by cetylpyridinium chloride. (2) High molecular weight material which is non-dialysable and precipitated by alcohol but not by cetylpyridinium chloride. (3) Low molecular weight material which is ultrafilterable and precipitated by alcohol and by cetylpyridinium chloride. (4) Low molecular weight material which is ultrafilterable and precipitated by alcohol but not by cetyipyridinium chloride. The heavy losses of glycosaminoglycans on dialysis have led us to conclude that this method should not be used to study the excretion of these polymers and that direct precipitation with cetylpyridinium chloride, which can be used to isolate both non-dialysable and ultrafilterable macromolecular fractions relevant to the diagnosis of the mucopolysaccharidoses, is the method of choice. |
| | |
Authors:
|
C A Pennock; R G Charles; D Stansbie |
Related Documents
:
|
7544465 - Subcutaneous injections of the tachykinin senktide reduce alcohol intake in alcohol-pre... 17188345 - The regulation of alcohol intake by melanin-concentrating hormone in rats. 97675 - Alcohol self-administration in monkeys (macaca radiata): the effects of prior alcohol e... 1252395 - The effect of sodium chloride ingestion on food intake and on fat deposition in male rats. 12298285 - Does a polyphagous caterpillar have the same gut microbiota when feeding on different s... 10765415 - Factors in exposure assessment: ethnic and socioeconomic differences in fishing and con... |
Publication Detail:
|
Type: Comparative Study; Journal Article |
Journal Detail:
|
Title: Annals of clinical biochemistry Volume: 12 ISSN: 0004-5632 ISO Abbreviation: Ann. Clin. Biochem. Publication Date: 1975 Sep |
Date Detail:
|
Created Date: 2005-01-10 Completed Date: 2005-02-01 Revised Date: 2009-11-19 |
Medline Journal Info:
|
Nlm Unique ID: 0324055 Medline TA: Ann Clin Biochem Country: England |
Other Details:
|
Languages: eng Pagination: 207-11 Citation Subset: IM |
Affiliation:
|
Department of Child Health, University of Bristol. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Adult Cetylpyridinium / diagnostic use Chemical Precipitation Child, Preschool Dialysis Solutions / chemistry Ethanol / diagnostic use Filtration Gas Chromatography-Mass Spectrometry Glycosaminoglycans / urine* Hexosamines / urine Hexuronic Acids / urine Humans Infant Molecular Weight Mucopolysaccharidoses / urine Solutions / analysis Time Factors |
| Chemical | |
Reg. No./Substance:
|
0/Dialysis Solutions; 0/Glycosaminoglycans; 0/Hexosamines; 0/Hexuronic Acids; 0/Solutions; 64-17-5/Ethanol; 7773-52-6/Cetylpyridinium |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Evaluation of a kit (Thyrolute) for the combined determination of serum total thyroxine and sequenti...
Next Document: A sampling device for septum-sealed blood containers.