Document Detail

Glycoproteomics of milk: differences in sugar epitopes on human and bovine milk fat globule membranes.
MedLine Citation:
PMID:  18624397     Owner:  NLM     Status:  MEDLINE    
Oligosaccharides from human and bovine milk fat globule membranes were analyzed by LC-MS and LC-MS/MS. Global release of N-linked and O-linked oligosaccharides showed both to be highly sialylated, with bovine peak-lactating milk O-linked oligosaccharides presenting as mono- and disialylated core 1 oligosaccharides (Galbeta1-3GalNAcol), while human milk had core type 2 oligosaccharides (Galbeta1-3(GlcNAcbeta1-6)GalNAcol) with sialylation on the C-3 branch. The C-6 branch of these structures was extended with branched and unbranched N-acetyllactosamine units terminating in blood group H and Lewis type epitopes. These epitopes were also presented on the reducing terminus of the human, but not the bovine, N-linked oligosaccharides. The O-linked structures were found to be attached to the high molecular mass mucins isolated by agarose-polyacrylamide composite gel electrophoresis, where MUC1 and MUC4 were present. Analysis of bovine colostrum showed that O-linked core 2 oligosaccharides are present at the early stage (3 days after birth) but are down-regulated as lactation develops. This data indicates that human milk may provide different innate immune protection against pathogens compared to bovine milk, as evidenced by the presence of Lewis b epitope, a target for the Helicobacter pylori bacteria, on human, but not bovine, milk fat globule membrane mucins. In addition, non-mucin-type O-linked fucosylated oligosaccharides were found (NeuAc-Gal-GlcNAc1-3Fuc-ol in bovine milk and Gal-GlcNAc1-3Fuc-ol in human milk). The O-linked fucose structure in human milk is the first to our knowledge to be found on high molecular mass mucin-type molecules.
Nicole L Wilson; Leanne J Robinson; Anne Donnet; Lionel Bovetto; Nicolle H Packer; Niclas G Karlsson
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Publication Detail:
Type:  Journal Article     Date:  2008-07-15
Journal Detail:
Title:  Journal of proteome research     Volume:  7     ISSN:  1535-3893     ISO Abbreviation:  J. Proteome Res.     Publication Date:  2008 Sep 
Date Detail:
Created Date:  2008-09-05     Completed Date:  2008-11-04     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101128775     Medline TA:  J Proteome Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3687-96     Citation Subset:  IM    
Proteome Systems Limited, Locked Bag 2073, North Ryde, Sydney, NSW 1670, Australia.
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MeSH Terms
Chromatography, Liquid
Electrophoresis, Agar Gel
Electrophoresis, Polyacrylamide Gel
Epitopes / chemistry*
Fats / analysis*
Milk / chemistry*
Mucins / analysis
Oligosaccharides / chemistry*
Tandem Mass Spectrometry
Reg. No./Substance:
0/Epitopes; 0/Fats; 0/Mucins; 0/Oligosaccharides

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