| Glycoprotein E of varicella-zoster virus enhances cell-cell contact in polarized epithelial cells. | |
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MedLine Citation:
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PMID: 11070038 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Varicella-zoster virus (VZV) infection involves the cell-cell spread of virions, but how viral proteins interact with the host cell membranes that comprise intercellular junctions is not known. Madin-Darby canine kidney (MDCK) cells were constructed to express the glycoproteins gE, gI, or gE/gI constitutively and were used to examine the effects of these VZV glycoproteins in polarized epithelial cells. At low cell density, VZV gE induced partial tight junction (TJ) formation under low-calcium conditions, whether expressed alone or with gI. Although most VZV gE was intracellular, gE was also shown to colocalize with the TJ protein ZO-1 with or without concomitant expression of gI. Freeze fracture electron microscopy revealed normal TJ strand morphology in gE-expressing MDCK cells. Functionally, the expression of gE was associated with a marked acceleration in the establishment of maximum transepithelial electrical resistance (TER) in MDCK-gE cells; MDCK-gI and MDCK-gE/gI cells exhibited a similar pattern of early TER compared to MDCK cells, although peak resistances were lower than those of gE alone. VZV gE expression altered F-actin organization and lipid distribution, but coexpression of gI modulated these effects. Two regions of the gE ectodomain, amino acids (aa) 278 to 355 and aa 467 to 498, although lacking Ca(2+) binding motifs, exhibit similarities with corresponding regions of the cell adhesion molecules, E-cadherin and desmocollin. These observations suggest that VZV gE and gE/gI may contribute to viral pathogenesis by facilitating epithelial cell-cell contacts. |
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Authors:
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C Mo; E E Schneeberger; A M Arvin |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Journal of virology Volume: 74 ISSN: 0022-538X ISO Abbreviation: J. Virol. Publication Date: 2000 Dec |
Date Detail:
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Created Date: 2000-12-04 Completed Date: 2000-12-04 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 0113724 Medline TA: J Virol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 11377-87 Citation Subset: IM |
Affiliation:
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Department of Pediatrics, Stanford University School of Medicine, Stanford, California 94305, USA. cmo@cmgm.stanford.edu |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Actins
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analysis Amino Acid Sequence Animals Cadherins / chemistry Cell Communication* Cell Line Cell Polarity Cytoskeletal Proteins / chemistry Desmocollins Desmoplakins Dogs Electric Impedance Epithelial Cells / physiology Herpesvirus 3, Human / physiology* Lipids / analysis Molecular Sequence Data Sequence Homology, Amino Acid Tight Junctions Viral Envelope Proteins / physiology* |
| Grant Support | |
ID/Acronym/Agency:
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AI20459/AI/NIAID NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Actins; 0/Cadherins; 0/Cytoskeletal Proteins; 0/Desmocollins; 0/Desmoplakins; 0/Lipids; 0/Viral Envelope Proteins; 0/glycoprotein gp1, varicella-zoster virus |
| Comments/Corrections | |
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