Document Detail

[Glycopeptide-resistant enterococci carriage: Are actual isolation and identification techniques sufficient?].
MedLine Citation:
PMID:  19477606     Owner:  NLM     Status:  In-Data-Review    
AIM: We compare two GRE screening methods and evaluate a new kit associating multiplex PCR and hybridization (Génotype(®) Enterococcus, Hain Lifescience) on a panel of 448 samples collected over a 4-month period.
PATIENTS AND METHODS: The first method is based on direct inoculation of the sample; the second one involves a preliminary enrichment phase followed by molecular diagnosis allowing the identification of species of enterococci as well as glycopeptide resistance genes.
RESULTS: All the resistant strains were isolated using the enrichment technique. The incidence of GRE (VanA) carriage was 0,55% (two out of 362 patients, two out of 448 isolates) with two Enterococcus faecium VanA. Six Enterococcus gallinarum VanC1 and two Enterococcus casseliflavus VanC2/C3 were also isolated and identified. The main clinics concerned are intensive care and hematology. The two patients with E. faecium VanA had been previously given glycopeptides for 10 days. For three strains, the molecular method allowed to correct prior erroneous results based on rapid identification (RapidID32Strep V2.0).
CONCLUSION: The method using direct samples inoculation underestimates real incidence of GRE carriage. The performances of Génotype(®) Enterococcus molecular method, evaluated for other parameters using reference strains and DNA sequencing, offer new possibilities applicable to routine laboratory.
C Surcouf; M Fabre; V Enouf; S Cadé; C Soler; C Mac Nab; T Samson; V Foissaud
Publication Detail:
Type:  English Abstract; Journal Article     Date:  2009-05-27
Journal Detail:
Title:  Pathologie-biologie     Volume:  59     ISSN:  1768-3114     ISO Abbreviation:  Pathol. Biol.     Publication Date:  2011 Jun 
Date Detail:
Created Date:  2011-06-13     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0265365     Medline TA:  Pathol Biol (Paris)     Country:  France    
Other Details:
Languages:  fre     Pagination:  146-50     Citation Subset:  IM    
Copyright Information:
Copyright © 2009 Elsevier Masson SAS. All rights reserved.
Hôpital d'instruction des armées Percy, 101, avenue Henri-Barbusse, 92141 Clamart cedex, France.
Vernacular Title:
Portage d'entérocoques résistants aux glycopeptides : les techniques d'isolement et d'identification actuelles sont-elles suffisantes ?
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