Document Detail

Glycomics investigation into insulin action.
MedLine Citation:
PMID:  16473469     Owner:  NLM     Status:  MEDLINE    
Defects in glycosylation are becoming increasingly associated with a range of human diseases. In some cases, the disease is caused by the glycosylation defect, whereas in others, the aberrant glycosylation may be a consequence of the disease. The implementation of highly sensitive and rapid mass spectrometric screening strategies for profiling the glycans present in model biological systems is revealing valuable insights into disease phenotypes. In addition, glycan screening is proving useful in the analysis of knock-out mice where it is possible to assess the role of glycosyltransferases and glycosidases and what function they have at the cellular and whole organism level. In this study, we analysed the effect of insulin on the glycosylation of 3T3-L1 cells and the effect of insulin resistance on glycosylation in a mouse model. Transcription profiling of 3T3-L1 cells treated with and without insulin revealed expression changes of several glycogenes. In contrast, mass spectrometric screening analysis of the glycans from these cells revealed very similar profiles suggesting that any changes in glycosylation were most likely on specific proteins rather than a global phenomenon. A fat-fed versus carbohydrate-fed mouse insulin resistant model was analysed to test the consequences of chronic insulin resistance. Muscle and liver N-glycosylation profiles from these mice are reported.
Simon Parry; Dirk Hadaschik; Christine Blancher; Mande K Kumaran; Natalia Bochkina; Howard R Morris; Sylvia Richardson; Timothy J Aitman; Dominique Gauguier; Ken Siddle; James Scott; Anne Dell
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-01-10
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  1760     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  2006 Apr 
Date Detail:
Created Date:  2006-04-25     Completed Date:  2006-06-09     Revised Date:  2014-02-19    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  652-68     Citation Subset:  IM    
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MeSH Terms
3T3-L1 Cells
Gene Expression Profiling
Gene Expression Regulation / drug effects
Glycoproteins / analysis*
Glycosylation / drug effects
Insulin / pharmacology*
Insulin Resistance / genetics*
Liver / chemistry
Mice, Inbred Strains
Muscles / chemistry
Polysaccharides / analysis*
Proteomics / methods
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Transcription, Genetic / drug effects
Grant Support
MC_U120061454//Medical Research Council; //Wellcome Trust
Reg. No./Substance:
0/Glycoproteins; 0/Insulin; 0/Polysaccharides

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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