| Glycomics investigation into insulin action. | |
| | |
MedLine Citation:
|
PMID: 16473469 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Defects in glycosylation are becoming increasingly associated with a range of human diseases. In some cases, the disease is caused by the glycosylation defect, whereas in others, the aberrant glycosylation may be a consequence of the disease. The implementation of highly sensitive and rapid mass spectrometric screening strategies for profiling the glycans present in model biological systems is revealing valuable insights into disease phenotypes. In addition, glycan screening is proving useful in the analysis of knock-out mice where it is possible to assess the role of glycosyltransferases and glycosidases and what function they have at the cellular and whole organism level. In this study, we analysed the effect of insulin on the glycosylation of 3T3-L1 cells and the effect of insulin resistance on glycosylation in a mouse model. Transcription profiling of 3T3-L1 cells treated with and without insulin revealed expression changes of several glycogenes. In contrast, mass spectrometric screening analysis of the glycans from these cells revealed very similar profiles suggesting that any changes in glycosylation were most likely on specific proteins rather than a global phenomenon. A fat-fed versus carbohydrate-fed mouse insulin resistant model was analysed to test the consequences of chronic insulin resistance. Muscle and liver N-glycosylation profiles from these mice are reported. |
| | |
Authors:
|
Simon Parry; Dirk Hadaschik; Christine Blancher; Mande K Kumaran; Natalia Bochkina; Howard R Morris; Sylvia Richardson; Timothy J Aitman; Dominique Gauguier; Ken Siddle; James Scott; Anne Dell |
Related Documents
:
|
23496829 - Internet-enabled pulmonary rehabilitation and diabetes education in group settings at h... 23647939 - Prevention of obesity and diabetes in childbearing women. 23497559 - Role of self-care in management of diabetes mellitus. 23070559 - The epigenome and its role in diabetes. 23393669 - Global prevalence and future of diabetes mellitus. 23416469 - Food for thought: the role of appetitive peptides in age-related cognitive decline. 8335679 - Microregional distributions of glucose, lactate, atp and tissue ph in experimental tumo... 12219719 - Portable blood glucose meters as a means of monitoring blood glucose concentrations in ... 3034949 - In vitro study of cultured human insulinoma cells: evidence of abnormal sensitivity to ... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2006-01-10 |
Journal Detail:
|
Title: Biochimica et biophysica acta Volume: 1760 ISSN: 0006-3002 ISO Abbreviation: Biochim. Biophys. Acta Publication Date: 2006 Apr |
Date Detail:
|
Created Date: 2006-04-25 Completed Date: 2006-06-09 Revised Date: 2007-08-13 |
Medline Journal Info:
|
Nlm Unique ID: 0217513 Medline TA: Biochim Biophys Acta Country: Netherlands |
Other Details:
|
Languages: eng Pagination: 652-68 Citation Subset: IM |
Affiliation:
|
Division of Molecular Biosciences, Imperial College, London, South Kensington, UK. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
3T3-L1 Cells Animals Gene Expression Profiling Gene Expression Regulation / drug effects Glycoproteins / analysis* Glycosylation / drug effects Insulin / pharmacology* Insulin Resistance / genetics* Liver / chemistry Mice Mice, Inbred Strains Muscles / chemistry Polysaccharides / analysis* Proteomics / methods Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Transcription, Genetic / drug effects |
| Grant Support | |
ID/Acronym/Agency:
|
//Wellcome Trust |
| Chemical | |
Reg. No./Substance:
|
0/Glycoproteins; 0/Polysaccharides; 11061-68-0/Insulin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: The rabbit coccidium Eimeria piriformis: Selection of a precocious line and life-cycle study.
Next Document: Vinorelbine in the management of breast cancer: New perspectives, revived role in the era of targete...