Document Detail


Glycomic analysis of sialic acid linkages in glycans derived from blood serum glycoproteins.
MedLine Citation:
PMID:  20345175     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A number of alterations to the normal glycomic profile have been previously described for a number of diseases and disorders, thus underscoring the medical importance of studying the glycans associated with proteins present in biological samples. An important alteration in cancer progression is an increased level of alpha2,6-sialylation, which aids in increasing the metastatic potential of tumor cells. Here we report a glycomic method that selectively amidates alpha2,6-linked sialic acids, while those that are alpha2,3-linked undergo spontaneous lactonization. Following subsequent permethylation, MALDI-TOF MS analysis revealed that many sialylated glycans present on glycoproteins found in blood serum featured increased levels of alpha2,6-sialylation in breast cancer samples. On the basis of the altered ratios of alpha2,3-linked to alpha2,6-linked sialic acids, many of these glycans became diagnostically relevant when they did not act as such indicators when based on traditional glycomic profiling alone.
Authors:
William R Alley; Milos V Novotny
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Journal of proteome research     Volume:  9     ISSN:  1535-3907     ISO Abbreviation:  J. Proteome Res.     Publication Date:  2010 Jun 
Date Detail:
Created Date:  2010-08-16     Completed Date:  2010-11-22     Revised Date:  2014-09-24    
Medline Journal Info:
Nlm Unique ID:  101128775     Medline TA:  J Proteome Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3062-72     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Amides / chemistry,  metabolism
Analysis of Variance
Blood Proteins / chemistry*
Breast Neoplasms / blood,  metabolism
Female
Glycomics / methods*
Glycoproteins / blood*,  chemistry*,  metabolism
Humans
Isomerism
Lactones / chemistry,  metabolism
Methylation
N-Acetylneuraminic Acid / chemistry*,  metabolism
Reproducibility of Results
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Grant Support
ID/Acronym/Agency:
GM24349/GM/NIGMS NIH HHS; P41 RR018942/RR/NCRR NIH HHS; P41 RR018942-03/RR/NCRR NIH HHS; R01 GM024349/GM/NIGMS NIH HHS; R01 GM024349-25/GM/NIGMS NIH HHS; RR018942/RR/NCRR NIH HHS; U01 CA128535/CA/NCI NIH HHS; U01 CA128535-03/CA/NCI NIH HHS; U01 CA128535-03/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Amides; 0/Blood Proteins; 0/Glycoproteins; 0/Lactones; GZP2782OP0/N-Acetylneuraminic Acid
Comments/Corrections

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