| Glycomic analysis of sialic acid linkages in glycans derived from blood serum glycoproteins. | |
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MedLine Citation:
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PMID: 20345175 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A number of alterations to the normal glycomic profile have been previously described for a number of diseases and disorders, thus underscoring the medical importance of studying the glycans associated with proteins present in biological samples. An important alteration in cancer progression is an increased level of alpha2,6-sialylation, which aids in increasing the metastatic potential of tumor cells. Here we report a glycomic method that selectively amidates alpha2,6-linked sialic acids, while those that are alpha2,3-linked undergo spontaneous lactonization. Following subsequent permethylation, MALDI-TOF MS analysis revealed that many sialylated glycans present on glycoproteins found in blood serum featured increased levels of alpha2,6-sialylation in breast cancer samples. On the basis of the altered ratios of alpha2,3-linked to alpha2,6-linked sialic acids, many of these glycans became diagnostically relevant when they did not act as such indicators when based on traditional glycomic profiling alone. |
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Authors:
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William R Alley; Milos V Novotny |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural |
Journal Detail:
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Title: Journal of proteome research Volume: 9 ISSN: 1535-3907 ISO Abbreviation: J. Proteome Res. Publication Date: 2010 Jun |
Date Detail:
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Created Date: 2010-08-16 Completed Date: 2010-11-22 Revised Date: 2011-07-28 |
Medline Journal Info:
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Nlm Unique ID: 101128775 Medline TA: J Proteome Res Country: United States |
Other Details:
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Languages: eng Pagination: 3062-72 Citation Subset: IM |
Affiliation:
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National Center for Glycomics and Glycoproteomics, Department of Chemistry, Indiana University, Bloomington, Indiana 47405, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amides
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chemistry,
metabolism Analysis of Variance Blood Proteins / chemistry* Breast Neoplasms / blood, metabolism Female Glycomics / methods* Glycoproteins / blood*, chemistry*, metabolism Humans Isomerism Lactones / chemistry, metabolism Methylation N-Acetylneuraminic Acid / chemistry*, metabolism Reproducibility of Results Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization |
| Grant Support | |
ID/Acronym/Agency:
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GM24349/GM/NIGMS NIH HHS; P41 RR018942-03/RR/NCRR NIH HHS; R01 GM024349-25/GM/NIGMS NIH HHS; RR018942/RR/NCRR NIH HHS; U01 CA128535-03/CA/NCI NIH HHS; U01 CA128535-03/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Amides; 0/Blood Proteins; 0/Glycoproteins; 0/Lactones; 131-48-6/N-Acetylneuraminic Acid |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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