Document Detail

Glycolipids: receptors for fibronectin?
MedLine Citation:
PMID:  6271797     Owner:  NLM     Status:  MEDLINE    
We have examined the hypothesis that glycolipids might serve as receptors for the cell surface glycoprotein fibronectin using three different biological assay systems. We find that purified solubilized gangliosides inhibit fibronectin-mediated hemagglutination, cell spreading, and restoration of a normal morphologic phenotype to transformed cells. The inhibition is dose-dependent and competitive; hemagglutination by 2 micrograms/ml fibronectin is half-maximally inhibited by less than 1 microM gangliosides. The most effective ganglioside inhibitors generally contain the most sialic acid residues. The isolated oligosaccharide portions of gangliosides retain this inhibitory activity and the oligosaccharides with more sialic acid are more effective inhibitors. A series of other lipids or ganglioside constituents are either less effective or without detectable activity. The more active of these lipids are the more negatively charged phospholipids such as phosphatidyl serine and phosphatidyl inositol. Our results support the hypothesis that the "receptors" for fibronectin on the cell surface either consist of or contain gangliosides or other negatively charged lipids.
K M Yamada; D W Kennedy; G R Grotendorst; T Momoi
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of cellular physiology     Volume:  109     ISSN:  0021-9541     ISO Abbreviation:  J. Cell. Physiol.     Publication Date:  1981 Nov 
Date Detail:
Created Date:  1982-01-28     Completed Date:  1982-01-28     Revised Date:  2005-11-17    
Medline Journal Info:
Nlm Unique ID:  0050222     Medline TA:  J Cell Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  343-51     Citation Subset:  IM    
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MeSH Terms
Biological Assay
Cell Line
Cell Movement / drug effects
Dose-Response Relationship, Drug
Fibronectins / metabolism*,  pharmacology
Gangliosides / metabolism*,  pharmacology
Hemagglutination / drug effects
Receptors, Cell Surface / analysis*
Receptors, Fibronectin
Reg. No./Substance:
0/Fibronectins; 0/Gangliosides; 0/Receptors, Cell Surface; 0/Receptors, Fibronectin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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