| Glycol methacrylate as an embedding medium for bone. | |
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MedLine Citation:
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PMID: 3590236 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A simple and reliable procedure for embedding undecalcified trabecular bone tissue in noncommercial glycol methacrylate (GMA) has been developed. The embedding mixture includes a monomer, methacrylic acid hydroxyethyl ester; a copolymer, methacrylic acid butyl ester; a cross-linker, ethylene glycol dimethacrylate; a catalyst, Luperco; a chemical initiator (N,N-dimethylaniline) and, to avoid excessive elevation of temperature during polymerization, a heat moderator, alpha-terpinene. The appropriate proportions of these components have been selected to give specimens which can be easily sectioned with classical microtomes and which do not swell but spread evenly on a water surface. Since polymerization occurs at -4 C, the method allows demonstration of such enzymatic activities as acid and alkaline phosphatase and carbonic anhydrase. It provides excellent preservation of bone tissue and in studies of bone metabolism allows histomorphometry as well as visualization of fluorescent labeling and radioactive markers. The cost is significantly less than available commercial kits. In our hands glycol methacrylate is at present more useful than methyl methacrylate and is used in our laboratory for routine embedding of bone tissue. |
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Authors:
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M Hott; P J Marie |
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Publication Detail:
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Type: Comparative Study; Journal Article |
Journal Detail:
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Title: Stain technology Volume: 62 ISSN: 0038-9153 ISO Abbreviation: Stain Technol Publication Date: 1987 Jan |
Date Detail:
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Created Date: 1987-07-09 Completed Date: 1987-07-09 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0404535 Medline TA: Stain Technol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 51-7 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Acrylates
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pharmacology* Animals Autoradiography Bone and Bones / anatomy & histology* Humans Ilium / anatomy & histology Methacrylates / pharmacology* Microscopy, Fluorescence Tissue Preservation / methods* |
| Chemical | |
Reg. No./Substance:
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0/Acrylates; 0/Methacrylates; 868-77-9/hydroxyethyl methacrylate |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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