Document Detail


Glycol methacrylate as an embedding medium for bone.
MedLine Citation:
PMID:  3590236     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A simple and reliable procedure for embedding undecalcified trabecular bone tissue in noncommercial glycol methacrylate (GMA) has been developed. The embedding mixture includes a monomer, methacrylic acid hydroxyethyl ester; a copolymer, methacrylic acid butyl ester; a cross-linker, ethylene glycol dimethacrylate; a catalyst, Luperco; a chemical initiator (N,N-dimethylaniline) and, to avoid excessive elevation of temperature during polymerization, a heat moderator, alpha-terpinene. The appropriate proportions of these components have been selected to give specimens which can be easily sectioned with classical microtomes and which do not swell but spread evenly on a water surface. Since polymerization occurs at -4 C, the method allows demonstration of such enzymatic activities as acid and alkaline phosphatase and carbonic anhydrase. It provides excellent preservation of bone tissue and in studies of bone metabolism allows histomorphometry as well as visualization of fluorescent labeling and radioactive markers. The cost is significantly less than available commercial kits. In our hands glycol methacrylate is at present more useful than methyl methacrylate and is used in our laboratory for routine embedding of bone tissue.
Authors:
M Hott; P J Marie
Related Documents :
7612216 - Molecular cloning, expression and evaluation of phosphohydrolases for phytate-degrading...
2334436 - Purification and n-terminal amino acid sequence of the tartrate-resistant acid phosphat...
2822006 - Proteolytic processing of chromogranin a in purified insulin granules. formation of a 2...
10417796 - Desmin phosphorylation abnormalities in cytoplasmic body and desmin-related myopathies.
2332106 - Inhibition of nuclear t3 binding by fatty acids: dependence on chain length, unsaturate...
2121536 - A novel method of preparing totally alpha-deuterated amino acids for selective incorpor...
Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Stain technology     Volume:  62     ISSN:  0038-9153     ISO Abbreviation:  Stain Technol     Publication Date:  1987 Jan 
Date Detail:
Created Date:  1987-07-09     Completed Date:  1987-07-09     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0404535     Medline TA:  Stain Technol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  51-7     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Acrylates / pharmacology*
Animals
Autoradiography
Bone and Bones / anatomy & histology*
Humans
Ilium / anatomy & histology
Methacrylates / pharmacology*
Microscopy, Fluorescence
Tissue Preservation / methods*
Chemical
Reg. No./Substance:
0/Acrylates; 0/Methacrylates; 868-77-9/hydroxyethyl methacrylate

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Some new methods for affixing sections to glass slides. I. Aqueous adhesives.
Next Document:  Synthesis and characteristics of allylic 4-pregnene-3,20-diols found in gonadal and breast tissues.